[Histonet] tissue does not sink in sucrose

[Emily Sours]

If your tissue is large, it might not sink in one night.  Leave it for
a few days.

Emily

On Jan 18, 2008 3:45 AM, Yves Heremans <Yves.Heremans <@t> vub.ac.be> wrote:
> Dear Histonetters,
>
> I am cryoprotecting mouse pancreas tissue (after fixation with
> formalin) in 30% sucrose in PBS. After overnight incubation in
> sucrose (at 4°C) some tissues sink, others do not. Is there a reason
> (and remedy) for this ?
>
> Thanks in advance,
>
> Yves Heremans
>
> Brussels Free University - VUB
> Diabetes Research Center
> Building D Level 2 Room D258
> Laarbeeklaan 103
> 1090 Brussels, Belgium
> Phone: +32 (0)2 477 44 73
> www.betacelltherapy.org
>
>
>
>
>
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