[Histonet] tissue does not sink in sucrose

[Yves Heremans]

Dear Histonetters,

I am cryoprotecting mouse pancreas tissue (after fixation with  
formalin) in 30% sucrose in PBS. After overnight incubation in  
sucrose (at 4°C) some tissues sink, others do not. Is there a reason  
(and remedy) for this ?

Thanks in advance,

Yves Heremans

Brussels Free University - VUB
Diabetes Research Center
Building D Level 2 Room D258
Laarbeeklaan 103
1090 Brussels, Belgium
Phone: +32 (0)2 477 44 73
www.betacelltherapy.org