[Histonet] Nuclear Staining on Prostate Biopsies

Joe Nocito jnocito <@t> satx.rr.com
Wed Jan 16 17:58:28 CST 2008

at my last place, we had to set up a different staining program for the 
reasons you stated. We decreased the hematoxylin and differentiation times, 
increased the bluing time and increased the wash time. I set up testing as 

Group 1: hema  at 30 second intervals 2:00, 2:30, 3:00, 3:30 , everything 
else stayed the same (4 slides)
Group 2 : hema  at 30 second intervals , but increase bluing 15 second 
intervals 30, 45, 60, 75 seconds, everything else stayed the same (16 
Group 3: hema 30 second intervals, bluing 15 second intervals and 
differentiation time by 15 seconds (64 slides, I think)

Lot of work, no doubt, but that's what the pathologists wanted. Have fun and 
good luck


----- Original Message ----- 
From: "Laurie Elmgren" <lelmgren <@t> sunriselab.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, January 16, 2008 11:42 AM
Subject: [Histonet] Nuclear Staining on Prostate Biopsies

I would like to optimize the quality of our stain.  We process office
biopsies, a good variety of tissue types.

The stain is consistently very good. There are three colors of Eosin,
and good nuclear detail on most tissue types, except for the prostate

The nuclei are stained dark and are "flat" having no depth.

I am thinking that it is not the fixation, because our Monday specimens
that are held over a full day are no different than those throughout the

Any suggestions before I start reprogramming the stainer?

Laurie Elmgren

Histology Supervisor

Sunrise Medical Labs

240 Motor Pkwy

Hauppauge, NY 11788


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