[Histonet] Tissue marking dyes and IHC

Ford, Judi judi.ford <@t> roche.com
Thu Jan 10 17:18:53 CST 2008

Hi all,

I was just curious to find out if tissue marked with either hematoxylin
and/or eosin, or tissue marking dyes, can that affect future IHC and/or
immunofluorescent staining? We have a situation where a right and left
piece of tissue will be embedded on one block and we want to distinguish
the two pieces. They are very small DRG's which can get lost once
embedded. One thought was to use dyes to mark them, but I was concerned
about the possibility of interference with immuno staining.

I'd love to hear your thoughts.

Thanks soooo much! 

Judi Ford, BA, HTL(ASCP)
Research Associate III
Roche Palo Alto
3431 Hillview Ave
Palo Alto, CA 94304

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