[Histonet] immunohistochemical detection of estrogen receptor
ratan choudhary
drratanji <@t> gmail.com
Thu Jan 10 12:33:06 CST 2008
Hi,
I want to start detection of estrogen receptor using Qdots goat anti
rabbit secondary body. I am new to use qdots. Please suggest me dilution
rate and other crucial steps.
Thanks
Ratan
On 1/10/08, histonet-request <@t> lists.utsouthwestern.edu <
histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. RE: Response to humor differences, etc. (Edwards, R.E.)
> 2. Agree to disagree (Webb, Dorothy L)
> 3. RE: Mart-1/Melan-A as a stain for melanoma sentinel nodes
> (Dawson, Glen)
> 4. Re: Who can perform Histology Duties (Rene J Buesa)
> 5. Mohs Tech position in Georgia (Shirley Powell)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 10 Jan 2008 17:09:07 -0000
> From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
> Subject: RE: [Histonet] Response to humor differences, etc.
> To: "Kathleen Roberts" <kgrobert <@t> rci.rutgers.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DC88BEDFD1FC3F468D0376A7C75465F7101C749E <@t> Saffron.cfs.le.ac.uk>
> Content-Type: text/plain; charset="us-ascii"
>
> "Emoticons", weren't they the baddies in an early episode of Dr.
> Who?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kathleen
> Roberts
> Sent: 10 January 2008 16:59
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: Re: [Histonet] Response to humor differences, etc.
>
> Actually, in my experience, it isn't just a Histonet thing, it's an
> ONLINE thing. No matter what forum or list you're on, there is always
> misunderstanding, fights and flaming b/c two crucial things are missing
> in online forums: tone of voice and facial expressions. (Yes, even when
> those are present, misunderstandings, etc. still occur.) Emoticons
> don't even begin to cut it, and sometimes when a person is reading
> something, his/her mood, opinions, experience, etc. will "color" what's
> being read and thus influence the person's understanding of what was
> written, which the author never intended and can't control. I can't
> tell you how many times that has happened to me.
>
> Kathleen Roberts
>
> Dawson, Glen wrote:
>
> >Humor IS shared by both Americans and British, as is misinterpretation,
> misunderstanding and becoming indignant/offended over the strangest
> things.
> >
> >It isn't an Americans vs. the British thing, it's a histonet thing.
> Over-reactions are rampant and one should be aware that having a sense
> of humor should have a "Use at your own risk" sign when utilizing it on
> this forum. In every group there will always be a number of folks more
> interested in attacking what is being said by others rather than
> contributing something of their own.
> >
> >Each year this trend gets a bit worse & I find myself "replying to all"
> less and less. Posting to an individual usually causes less of a stir
> so that is the road I take much more these days.
> >
> >Just my .01 Euros,
> >
> >Glen Dawson
> >IHC Manager
> >Milwaukee, WI
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Kemlo
> >Rogerson
> >Sent: Thursday, January 10, 2008 8:44 AM
> >To: Robyn Vazquez; MICHELLE.MCNEESE <@t> childrens.com;
> >histonet <@t> lists.utsouthwestern.edu; cmiller <@t> physlab.com; JWEEMS <@t> sjha.org
> >Subject: RE: [Histonet] Response to On-Call pay
> >
> >
> >It's the humour Robyn, rarely travels well, I sometimes wonder if the
> >difference between Americans and the British is greater than that
> >between men and women.
> >
> >
> >Kemlo Rogerson
> >Pathology Manager
> >DD 01934 647057 or extension 3311
> >Mob 07749 754194; Pager 07659 597107;
> >Don't be afraid to take a big step when one is indicated. You can't
> >cross a chasm in two small jumps. --Buckminster Fuller
> >
> >This e-mail is confidential and privileged. If you are not the intended
>
> >recipient please accept my apologies; please do not disclose, copy or
> >distribute information in this e-mail or take any action in reliance on
>
> >its contents: to do so is strictly prohibited and may be unlawful.
> >Please inform me that this message has gone astray before deleting it.
> >Thank you for your co-operation
> >
> >
> >
> >_______________________________________________
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> >
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>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 10 Jan 2008 11:19:29 -0600
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] Agree to disagree
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <0E394B648E5284478A6CCB78E5AFDA27056353B9 <@t> hpes1.HealthPartners.int>
> Content-Type: text/plain; charset="us-ascii"
>
> I agree with Glen inasmuch as I do not do a lot of "post" responses due
> to being attacked! Constructive criticism is great and offering gentle
> advice is even better, but not "putting one down" because they do not
> know as much as someone else may or have a different viewpoint!! I
> still LOVE the histonet and appreciate all of the great wisdom instilled
> in me by those of you who keep responding to my inquiries and others!!
>
> Dorothy Webb, HT (ASCP)
> Histology Technical Supervisor
> Regions Hospital, Pathology Department
> 640 Jackson Street, Saint Paul, MN 55101-2595
> Phone: 651-254-2962
> Fax: 651-254-2741
> Regions Hospital is part of the HealthPartners family of care
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> dissemination, forwarding, printing, or copying of this e-mail is strictly
> prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will be
> reimbursed for reasonable costs incurred in notifying us.
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 10 Jan 2008 11:23:52 -0600
> From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
> Subject: RE: [Histonet] Mart-1/Melan-A as a stain for melanoma
> sentinel nodes
> To: "Barnhart, Tammy" <Tbarnhart <@t> primecare.org>, "Histonet
> \(E-mail\)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> B3D65550856D0146B900D401EE313D4B01274146 <@t> dynams.dynacaremilwaukee.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Tammy,
>
> I use a cocktail of Mart-1, Melan-A & Tyrosinase that we named the MCW
> (Medical College of Wisconsin) Melanoma Cocktail. If you google it you
> should find our paper online and I do believe that my Pathologist addresses
> some Mast cell staining issues in it. The triple antibody is excellent as a
> screener as it covers more epitopes than any one of the three does
> individually. Suggested staining methods are in the paper as well.
>
> Best of Luck,
> Glen Dawson
> IHC Manager
> Milwaukee, WI
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Barnhart,
> Tammy
> Sent: Thursday, January 10, 2008 10:51 AM
> To: Histonet (E-mail)
> Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel
> nodes
>
>
> We are having some debate about which marker to use for our melanoma
> sentinel node screens. We have been using Mart-1, but recently had a case
> where we think mast cells were stained. We can find no literature that
> indicates that Mart-1 will stain mast cells. We use Lab Vision's
> Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to
> see what stained, and all the cells that stained with the Mart-1 stained
> plus a bunch of others. So if the Mart-1 is staining mast cells, it is
> only
> staining a subset of them. We really think that these are mast cells and
> not melanoma, but would like to see some literature to support this. So
> two
> questions: 1) What are people using as a screening antibody and 2) Does
> anyone have a reference that indicates that this antibody will stain mast
> cells. Thanks in advance for your help.
>
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 10 Jan 2008 09:41:38 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Who can perform Histology Duties
> To: Terri Braud <tbraud <@t> holyredeemer.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <463239.48520.qm <@t> web61219.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> In the same way a "well trained woo-doo priest" can perform surgery? What
> about the knowledge background? Irrelevant?
> René J.
>
> Terri Braud <tbraud <@t> holyredeemer.com> wrote:
>
>
>
>
> ---------------------------------
> Looking for last minute shopping deals? Find them fast with Yahoo!
> Search.
>
> ------------------------------
>
> Message: 5
> Date: Thu, 10 Jan 2008 12:52:13 -0500
> From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
> Subject: [Histonet] Mohs Tech position in Georgia
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <01MPXG4PEYQE003MTI <@t> Macon2.Mercer.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Please Do Not reply to me. I am posting this for a friend.
> Please contact the Dr. Lane or Renee Blanton at the numbers below.
>
> Needed immediately
> Certified Mohs histotech
>
> Exciting new Mohs surgery practice in Columbus, Georgia
>
> Part-time or full-time possible
>
> Competitive pay
> Excellent benefits
>
> Contact: Joshua Lane, M.D. at 706.573.6545 or Renee Blanton at
> 706.322.1717
>
>
>
>
> ------------------------------
>
> _______________________________________________
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>
> End of Histonet Digest, Vol 50, Issue 15
> ****************************************
>
--
***********************************************
Ratan Kumar Choudhary
Graduate Research Assistant
Bovine Functional Genomics Lab
Room 14
Bldg 200 BARC-East
Powder Mill Road
Beltsville, Maryland, 20705
USA
Phone: 301-504-8342 (O)
301-931-8730 (R)
Fax: (301) 504-8414
http://www.drratankumar.wetpaint.com
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