[Histonet] decal mouse feet & IHC

Gayle Callis gayle.callis <@t> bresnan.net
Tue Jan 8 15:48:26 CST 2008

If the antigen is not damaged by acids, then buffered formic acid should 
work using endpoint determination to ensure no overexposure to the acid. 
Normally we do 14% tetrasodium EDTA in Dulbeccos PBS at pH 7.4 - 7.6, the pH 
range of TRIS buffered saline.  WE do adjust the pH down with glacial acetic 
acid using constant stirring and a pH meter.  The decalcification may take a 
week or more, once again tested with endpoint determination, the weight 
gain/weight loss method or FAXITRON xray method.

Mouse paws take longer to fix with NBF than you think, unless you start with 
perfusion fixation followed by immersion.  We have fixed for up to a week 
since the bones are very packed very closed together with skin and tendons 

Gayle M. Callis

----- Original Message ----- 
From: "Helen E Johnson" <hej01 <@t> health.state.ny.us>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 08, 2008 12:51 PM
Subject: [Histonet] decal mouse feet & IHC

> Hi Histonetters,
>     How are you decaling mouse feet for IHC?
>                                Helen Johnson (hej01 <@t> health.state.ny.us)

More information about the Histonet mailing list