[Histonet] Dako Autostainer plus issues

[Liz Chlipala]

Ben

We use a pap pen all the time on the dako autostainer.  We place our
tissues on the lower portion of the slides so we are primarily using
drop zones 1 and 2.  If the tissue is small enought that one drop zone
will cover it we will use drop zone one with a volume of 150ul.  If the
tissue spans 2 drop zones we use drop zones 1 and 2 with a volume of
100ul.  And if we have tissue that covers the entire slide, we use 3
drop zones with 100ul per drop zone.  We place the pap pen above the
tissue just one line, occasionly we get slides in from clients that have
tissues placed all over the slide or in the middle, we will then use
drop zones 2 or 3 and place a pap pen line on the top and bottom of the
tissue.  This works well for us. Just make sure that the pap pen line is
not too close to the tissue.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kriederman, Ben
Sent: Tuesday, January 08, 2008 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dako Autostainer plus issues

Hi all,
 
Has anyone used the pap pen with the dako autostainer plus to avoid the
fluid from shrinking up on the slides and exposing dry portions of
tissue?  If so what has been your experience with the pen.  What volumes
do you use in the drop zones of your staining protocols?
 
Thanks for the help,
 
Ben


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