[Histonet] c-kit (CD117) Ab & Protocol needed - 4%PFA Frozen Mouse Testes Sections

[Dana Settembre]

Hello Donna,
I am sorry I use c-kit on human paraffin tissue and if it might help
I use  Cell Marque's c-kit  cat#  CMD768 @ 1:100 dilution and
Dako's Env+ Rabbit detection kit.
I use Triliogy HIER, but as I stated I use it on paraffin section.
I wouldn't use it on frozens.

Good Luck,


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> <djemge <@t> aol.com> 01/07/08 3:23 PM >>>


Hello Histonetters,
Please share a?working c-kit ab (company, cat. #, dilution) and
detailed protocol used in your lab for 4%PFA,?30% sucrose cryoprotected,
Frozen Mouse Testes sections.?We have been using an ab by Santa Cruz and
haven't had much success on this tissue. I have tried our Ab at
different dilutions, with 0.3% H202 quenching, with and w/out sodium
citrate HEIR, blocked with goat serum. So far no luck or lots of
background. Help!

Donna 

Donna Emge, HT-ASCP
Northwestern University
303 E. Superior, Lurie 7-220
Chicago, IL 60611
312-503-2036
d-emge <@t> northwestern.edu 

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