[Histonet] cracked samples

Anthony Gatt Anthony.Gatt <@t> jefferson.edu
Tue Jan 8 07:58:44 CST 2008


Hello,
I have been paraffin sectioning drosophila heads at 6 microns.  After H&E staining and observing them under a microscope, I notice that some sections have cracks in them.  Sometimes the cracks are so bad that the sample is unusable.  However, often in the same block some of the samples are perfect.  Any ideas?
Thanks.



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