[Histonet] k-9 Vet Plasma Marker

[Jan Shivers]

I use canine IgG, IgM, and IgA.  Dilutions will vary, depending upon 
antibody source and stock immunoglobulin concentrations, plus whatever 
detection system, incubation times and temperatures that you use.  Positive 
controls are dog tonsil for IgG and IgM; dog stomach or pylorus for IgA.

Proteinase K enzyme digestion for 5'.

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Leader
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

----- Original Message ----- 
From: "Bliven, Laura" <bliven.laura <@t> marshfieldclinic.org>
To: <histonet <@t> lists.utsouthwestern.edu>; <ihcrg <@t> googlegroups.com>
Sent: Thursday, January 03, 2008 9:41 AM
Subject: [Histonet] k-9 Vet Plasma Marker


> What's the best plasma cell marker for tumors on dog tissue?
> Additional info would be appreciated as to antigen retrieval and dilution, 
> even though we'll be testing out various steps.
> Thanks,
> Laura
>
> Laura Bliven
> Histology Lab/IHC
> Marshfield Laboratories
> 1000 N. Oak Ave.
> Marshfield, WI 54449
>
>
>
>


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