[Histonet] CD68

Wynn, Carmen Carmen.Wynn <@t> us.astellas.com
Thu Jan 3 10:26:06 CST 2008


Hello,

I have had good results with AbD Serotec's CD68 (cat# MCA1957) on frozen
acetone fixed mouse heart allografts with 2-step indirect
immunohistochemical staining.  You should probably titrate to a suitable
dilution.  I have not tried this antibody on paraffin sections.   

Good Luck,

Carmen Wynn, M.S., Senior Scientist
Astellas Research Institute of America, LLC. (ARIA)
Illinois Science and Technology Park
8045 Lamon Ave
Skokie, IL 60077
Direct: 847-933-7419
Main: 847-933-7400
Fax: 847-933-7401 


-----Original Message-----
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[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, December 20, 2007 5:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 49, Issue 22

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Today's Topics:

   1. IHC Technologist Position at PhenoPath (Seattle, WA)
      (Owen, Michael P)
   2. Re: Osmometer (Robert Chiovetti)
   3. Re: CD68 (Majid ghoddusi)
   4. Happy Holidays! (arnie.jimenez <@t> vel-lab.com)
   5. RE: CD68 (Josh Wray)
   6. MICROWAVES (Janice Mitchell)
   7. Off Topic:  NO SANTA CLAUS  (Ingles Claire)
   8. RE: Off Topic:  NO SANTA CLAUS  (Thomas Jasper)
   9. RE: Off Topic:  NO SANTA CLAUS (Nancy  Lemke)
  10. Tissue Tek anti-roll device (Shirley Powell)
  11. Re: MICROWAVES (Phil McArdle)
  12. Jacquelyn Grewe/Staff/OhioHealth is out of the office .
      (JGREWE <@t> OhioHealth.com)
  13. Re: Tissue Tek anti-roll device (Jennifer MacDonald)
  14. Christmas Greetings, and thanks to... (mtitford <@t> aol.com)
  15. IHC for VIAS (Godfrey Guerzon)
  16. RE: SPAM-LOW:  [Histonet] IHC for VIAS (Douglas D Deltour)
  17. MITF (Douglas D Deltour)
  18. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels)
  19. Re: IHC for VIAS (Patti Loykasek)
  20. IgA-FITC (Houston, Ronald)
  21. Off topic:  Free samples of chemiluminescent substrates
      (richardkuzma <@t> michdiag.com)
  22. Re: IHC for VIAS (Godfrey Guerzon)
  23. RE: IHC for VIAS (Annette Hall)


----------------------------------------------------------------------

Message: 1
Date: Thu, 20 Dec 2007 13:04:17 -0500
From: "Owen, Michael P" <michael.owen <@t> fda.hhs.gov>
Subject: [Histonet] IHC Technologist Position at PhenoPath (Seattle,
	WA)
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<449E51C6DA0AD840B44F57C7A6EB07BF0422035E <@t> FMD3VS022.fda.gov>
Content-Type: text/plain; charset=us-ascii

Immunohistochemistry (IHC) Technologist

craigslist Seattle-Tacoma / jobs / biotech and science
http://seattle.craigslist.org
http://seattle.craigslist.org/see/sci/514227920.html


------------------------------------------------------------------------
--------
Reply to: jobs <@t> phenopath.com
Date: 2007-12-19, 9:46AM PST


PhenoPath Laboratories, a national pathology reference laboratory, has
an opportunity in the clinical immunohistochemistry division for a
full-time IHC technologist, day shift. We are in a state-of-the-art
facility located in Seattle, WA. 

JOB DESCRIPTION: 
Responsibilities may include performing immunohistochemistry, and
immunofluorescence on patient samples and tissue sectioning (paraffin
and frozen). Participation in the development of new tests or
technologies, as well as participation in clinical research projects is
also included. Quality control, safety, and preventive maintenance
procedures and documentation are required elements of this position as
well. 

REQUIRED SKILLS/EXPERIENCE: 
Strong preference given to ASCP certified (or certification-eligible)
laboratory techs. Trained laboratory techs of any discipline are
encouraged to apply (histotech, med tech, cytotech). PhenoPath
Laboratories is committed to hiring the best person for the job. 
PhenoPath Laboratories offers a competitive compensation and benefits
package, including 401k.
 
TO APPLY, PLEASE E-MAIL OR FAX COMPLETED APPLICATION FOR EMPLOYMENT AND
RESUME TO: 
Paul Moore, Assistant to the Director of Human Resources PhenoPath
Laboratories 551 N. 34th St., Suite 100 Seattle, WA 98103 Fax: 206
374-9009 E-mail: jobs <@t> phenopath.com (Preferred method of application) No
phone calls about this job, please.  
  
Location: Seattle, WA 
Compensation: DOE 
Principals only. Recruiters, please don't contact this job poster. 
Please, no phone calls about this job! 
Please do not contact job poster about other services, products or
commercial interests.

PostingID: 514227920




Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE  Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail: michael.owen <@t> fda.hhs.gov




------------------------------

Message: 2
Date: Thu, 20 Dec 2007 10:11:16 -0800 (PST)
From: Robert Chiovetti <rchiovetti <@t> yahoo.com>
Subject: Re: [Histonet] Osmometer
To: Mauricio Avigdor <bitesizellama <@t> gmail.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <756859.64500.qm <@t> web58911.mail.re1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hi Mauricio,

Well, that's interesting.  The "more modern techniques" still require an
instrument to measure osmolality!  Maybe you need to ask someone else in
the laboratory...

There are two basic ways to measure osmolality:  vapor pressure and
freezing point depression.  In the vapor pressure instruments, you soak
a small filter paper circle with the solution, put it in a sealed
chamber and measure the amount of heat that is required to vaporize the
solution and form an amount of pressure in the chamber.  The freezing
point depression machines usually require a little more solution.  It's
put in a small chamber, the chamber is chilled, and the temperature is
measured at which the solution freezes.  There is sometimes a small
vibrating probe in the solution to agitate it while it's cooling down.

At least these are the two old standby methods.  There are probably more
freezing point depression osmometers in use than vapor pressure
osmometers, but they have both been around for many years!

Maybe you should ask someone else in another lab?

Cheers,

Bob
 
Robert (Bob) Chiovetti, Ph.D.
Southwest Precision Instruments
See What's New on Our Website!
Arizona's Microscopy Resource
132 North Elster Drive
Tucson, AZ 85710-3212
Tel./Fax 520-546-4986
Member, Arizona Small Business Association
(www.asba.com)

----- Original Message ----

I asked to borrow an osmometer from another lab, and was told that they
 no
longer use these, as they have moved on to "more modern techniques". I
 am
really curious to know what these techniques could be.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






 
________________________________________________________________________
____________
Be a better friend, newshound, and 
know-it-all with Yahoo! Mobile.  Try it now.
http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ 


------------------------------

Message: 3
Date: Thu, 20 Dec 2007 10:15:49 -0800
From: "Majid ghoddusi" <majid.ghoddusi <@t> gmail.com>
Subject: Re: [Histonet] CD68
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<f7650feb0712201015g53f91c77s8189cc44068ed9b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

While on CD 68 subject, I have been told that this does not work on
mouse
tissue. In other words it is not a good IHC marker for mouse
macrophages. I
would appreciate advice from people who have successfuly tried this on
mouse
tissue.

Thanks,

Majid






*Majid Ghoddusi, DVM, PhD*
*Veterinary Pathologist*
*Comparative Biosciences, Inc.*
*786 Lucerne Drive, *
*Sunnyvale, CA 94085*
*http://www.compbio.com/* <http://www.compbio.com/>
*Ph: 408-738-9265*
*Fax: 408-738-9278*




On Dec 20, 2007 9:58 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> IF you have both control and case running simultaneously and there is
no
> problem with the instrument (like malfunctioning over one slide and
not over
> the other, which is very unlikely) your problem is with your control.
>  I always used tonsils coming from surgey and ususally older than 2 yo
> patients.
>  I used Dako IgMo Ab at 1:1000 with pH6 HIER
>  Try a new control tissue (fresh sections if at all possible).
>  Ren J.
>
> Cheri Miller <cmiller <@t> physlab.com> wrote:
>
>
>
>
>
>
> ---------------------------------
> Looking for last minute shopping deals?  Find them fast with Yahoo!
> Search.
>  _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 4
Date: 20 Dec 2007 12:11:50 -0600
From: arnie.jimenez <@t> vel-lab.com
Subject: [Histonet] Happy Holidays!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<20071220181150.19040.qmail <@t> ux-vhost05.dllstx2.theplanet.com>
Content-Type: text/plain; charset="UTF-8"

This is an automated response.

Thank you for contacting Vel-Lab Research. 

We are shutdown for the holidays. We use this time to give our employees
a well deserved break, we also take the opportunity to thouroghly clean
our facilities and calibrate all our equipment. 
Your project is our top priority and we will return to it as soon as
possible. I will personally return to the lab Dec. 27th and will attend
to any urgent issues then. The full lab will be back the first week of
January. Thank you for your understanding.

Happy Holidays from everyone at Vel-Lab

Arnie Jimenez
Owner
Vel-Lab Research





------------------------------

Message: 5
Date: Thu, 20 Dec 2007 13:27:35 -0500
From: "Josh Wray" <jwray78 <@t> gmail.com>
Subject: [Histonet] RE: CD68
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<883927cf0712201027u1dc19f6o998092564f7eebec <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

 We too had a problem with tonsil for a CD68 control. We decided to run
a
lymph node as the QC. It worked out much better.




 Josh Wray HT(ASCP)

 Ameripath Indianapolis


------------------------------

Message: 6
Date: Thu, 20 Dec 2007 13:43:19 -0500
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] MICROWAVES
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s76a7186.054 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Cap requests "microwave devices be periodically monitored for
reproducibility" what is considered periodically?  
                      Thanks, Janice



------------------------------

Message: 7
Date: Thu, 20 Dec 2007 12:59:10 -0600
From: "Ingles Claire" <CIngles <@t> uwhealth.org>
Subject: [Histonet] Off Topic:  NO SANTA CLAUS 
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<08A0A863637F1349BBFD83A96B27A50A1200B7 <@t> uwhis-xchng3.uwhis.hosp.wisc.edu
>
	
Content-Type: text/plain;	charset="iso-8859-1"

 
Sorry if you are not Christian. You can delete, but I think Santa just
has lots of different names around the world. Everyone - remember what
you have that never cost anything. Merry Christmas greetings and warm
wishes to all.
 
Claire Ingles
lifelong Santa's helper

 

					  I remember my first Christmas
adventure with Grandma. 
					I was just a kid. 
					
					I remember tearing across town
on my bike to visit her on the day my big sister dropped the bomb: 
					
					"There is no Santa Claus," she
jeered. "Even dummies know that!" 
					
					My Grandma was not the gushy
kind, never had been. 
					
					I fled to her that day because I
knew she would be straight with me. I knew Grandma always told the
truth, and I knew that the truth always went down a whole lot easier
when swallowed with one of her worl d-famous cinnamon buns. 
					
					I knew they were world-famous,
because Grandma said so. 
					It had to be true. 
					Grandma was home, and the buns
were still warm. 
					
					Between bites, I told her
everything. She was ready for me. 
					"No Santa Claus! !" she snorted.
"Ridiculous! 
					" Don't believe it. That rumor
has been going around for years, and it makes me mad, plain mad.' 
					
					Now, put on your coat, and let's
go." 
					"Go? Go where, Grandma?" I
asked. 
					I hadn't even finished my second
world-famous, cinnamon bun. 
					
					"Where" turned out to be Kerby's
General Store, the one store in town that had a little bit of just about
everything. As we walked through its doors, Grandma handed me ten
dollars. That was a bundle in those days. 
					
					"Take this money," she said,
"and buy something for someone who needs it. I'll wait for you in the
car." 
					
					Then she turned and walked out
of Kerby's. I was only eight years old. I'd often gone shopping with my
mother, but never had I shopped for anything all by myself. 
					
					The store seemed big and
crowded, full of people scrambling to finish their Christmas shopping.
For a few moments I just stood there, confused, clutching that
ten-dollar bill, wondering what to buy, and who on earth to buy it for. 
					
					I thought of everybody I knew:
my family, my friends, my neighbors, the kids at school, the people who
went to my church. 
					
					I was just about thought out,
when I suddenly thought of Bobby Decker. He was a kid with bad breath
and messy hair, and he sat right behind me in Mrs. Pollock's grade-two
class. Bobby Decker didn't have a coat. 
					
					I knew that because he never
went out for recess during the winter. His mother always wrote a note,
telling the teacher that he had a cough, but all we kids knew that Bobby
Decker didn't have a cough, and he didn't have a coat. 
					
					I fingered the ten-dollar bill
with growing excitement. I would buy Bobby Decker a coat! I settled on a
red corduroy one that had a hood to it. It looked real warm, and he
would like that. 
					
					"Is this a Christmas present for
someone?" the lady behind the counter asked kindly, as I laid my ten
dollars down. 
					
					"Yes," I replied shyly. "It's
... for Bobby." 
					
					The nice lady smiled at me. I
didn't get any change, but she put the coat in a bag and wished me a
Merry Christmas. 
					
					That evening, Grandma helped me
wrap the coat in Christmas paper and ribbons (a little tag fell out of
the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From
Santa Claus", on a tag-- Grandma said that Santa always insisted on
secrecy. 
					
					Then she drove me over to Bobby
Decker's house, explaining as we went that I was now and forever
officially one of Santa's helpers. 
					
					Grandma parked down the street
from Bobby's house, and she and I crept noiselessly and hid in the
bushes by his front walk. Then Gr andma gave me a nudge. "All right,
Santa Claus," she whispered, "get going." 
					I took a deep breath, dashed for
his front door, and threw the present down on his step. I pounded his
door and flew back to the safety of the bushes and Grandma. 
					
					Together we waited breathlessly
in the darkness for the front door to open. Finally it did, and there
stood Bobby. Fifty years haven't dimmed the thrill of those moments
spent shivering beside my Grandma, in Bobby Decker's bushes. 
					
					That night, I realized that
those awful rumors about Santa Claus were just what Grandma said they
were: "Ridiculous". Santa was alive and well, and we were on his team. 
					
					I still have the Bible, with the
tag tucked inside: $19.95. 
					
	
-------------------------------------------------------------------- 
					He who has no Christmas in his
heart will never find Christmas under a tree. 
					
					Have a wonderful holiday season.
Merry Christmas and a Happy New Year!! 
					
					
					
					
					
					




------------------------------

Message: 8
Date: Thu, 20 Dec 2007 12:07:49 -0800
From: "Thomas Jasper" <tjasper <@t> copc.net>
Subject: RE: [Histonet] Off Topic:  NO SANTA CLAUS 
To: "Ingles Claire" <CIngles <@t> uwhealth.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<90354A475B420441B2A0396E5008D4965E201A <@t> copc-sbs.COPC.local>
Content-Type: text/plain; charset="us-ascii"

Thanks Claire, totally awesome.  Merry Christmas and Happy New Year from
one of Santa helpers to another.
Tom Jasper
Bend, OR (by way of northern WI)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Thursday, December 20, 2007 10:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Off Topic: NO SANTA CLAUS 

 
Sorry if you are not Christian. You can delete, but I think Santa just
has lots of different names around the world. Everyone - remember what
you have that never cost anything. Merry Christmas greetings and warm
wishes to all.
 
Claire Ingles
lifelong Santa's helper

 

					  I remember my first Christmas
adventure with Grandma. 
					I was just a kid. 
					
					I remember tearing across town
on my bike to visit her on the day my big sister dropped the bomb: 
					
					"There is no Santa Claus," she
jeered. "Even dummies know that!" 
					
					My Grandma was not the gushy
kind, never had been. 
					
					I fled to her that day because I
knew she would be straight with me. I knew Grandma always told the
truth, and I knew that the truth always went down a whole lot easier
when swallowed with one of her worl d-famous cinnamon buns. 
					
					I knew they were world-famous,
because Grandma said so. 
					It had to be true. 
					Grandma was home, and the buns
were still warm. 
					
					Between bites, I told her
everything. She was ready for me. 
					"No Santa Claus! !" she snorted.
"Ridiculous! 
					" Don't believe it. That rumor
has been going around for years, and it makes me mad, plain mad.' 
					
					Now, put on your coat, and let's
go." 
					"Go? Go where, Grandma?" I
asked. 
					I hadn't even finished my second
world-famous, cinnamon bun. 
					
					"Where" turned out to be Kerby's
General Store, the one store in town that had a little bit of just about
everything. As we walked through its doors, Grandma handed me ten
dollars. That was a bundle in those days. 
					
					"Take this money," she said,
"and buy something for someone who needs it. I'll wait for you in the
car." 
					
					Then she turned and walked out
of Kerby's. I was only eight years old. I'd often gone shopping with my
mother, but never had I shopped for anything all by myself. 
					
					The store seemed big and
crowded, full of people scrambling to finish their Christmas shopping.
For a few moments I just stood there, confused, clutching that
ten-dollar bill, wondering what to buy, and who on earth to buy it for. 
					
					I thought of everybody I knew:
my family, my friends, my neighbors, the kids at school, the people who
went to my church. 
					
					I was just about thought out,
when I suddenly thought of Bobby Decker. He was a kid with bad breath
and messy hair, and he sat right behind me in Mrs. Pollock's grade-two
class. Bobby Decker didn't have a coat. 
					
					I knew that because he never
went out for recess during the winter. His mother always wrote a note,
telling the teacher that he had a cough, but all we kids knew that Bobby
Decker didn't have a cough, and he didn't have a coat. 
					
					I fingered the ten-dollar bill
with growing excitement. I would buy Bobby Decker a coat! I settled on a
red corduroy one that had a hood to it. It looked real warm, and he
would like that. 
					
					"Is this a Christmas present for
someone?" the lady behind the counter asked kindly, as I laid my ten
dollars down. 
					
					"Yes," I replied shyly. "It's
... for Bobby." 
					
					The nice lady smiled at me. I
didn't get any change, but she put the coat in a bag and wished me a
Merry Christmas. 
					
					That evening, Grandma helped me
wrap the coat in Christmas paper and ribbons (a little tag fell out of
the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From
Santa Claus", on a tag-- Grandma said that Santa always insisted on
secrecy. 
					
					Then she drove me over to Bobby
Decker's house, explaining as we went that I was now and forever
officially one of Santa's helpers. 
					
					Grandma parked down the street
from Bobby's house, and she and I crept noiselessly and hid in the
bushes by his front walk. Then Gr andma gave me a nudge. "All right,
Santa Claus," she whispered, "get going." 
					I took a deep breath, dashed for
his front door, and threw the present down on his step. I pounded his
door and flew back to the safety of the bushes and Grandma. 
					
					Together we waited breathlessly
in the darkness for the front door to open. Finally it did, and there
stood Bobby. Fifty years haven't dimmed the thrill of those moments
spent shivering beside my Grandma, in Bobby Decker's bushes. 
					
					That night, I realized that
those awful rumors about Santa Claus were just what Grandma said they
were: "Ridiculous". Santa was alive and well, and we were on his team. 
					
					I still have the Bible, with the
tag tucked inside: $19.95. 
					
	
-------------------------------------------------------------------- 
					He who has no Christmas in his
heart will never find Christmas under a tree. 
					
					Have a wonderful holiday season.
Merry Christmas and a Happy New Year!! 
					
					
					
					
					
					


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 9
Date: Thu, 20 Dec 2007 15:22:11 -0500
From: "Nancy  Lemke" <nsnwl <@t> neuro.hfh.edu>
Subject: RE: [Histonet] Off Topic:  NO SANTA CLAUS
To: "Thomas Jasper" <tjasper <@t> copc.net>, "Ingles Claire"
	<CIngles <@t> uwhealth.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <bf30cbb820b3c4900df40baca9bf4dc8 <@t> neuro.hfh.edu>
Content-Type: text/plain; charset=us-ascii

Merry Christmas to you both and to all Santa's helpers, big and small!


Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Thomas Jasper" tjasper <@t> copc.net
Date: Thu, 20 Dec 2007 16:09:31 -0500
To: "Ingles Claire" CIngles <@t> uwhealth.org
Subject: RE: [Histonet] Off Topic:  NO SANTA CLAUS

> Thanks Claire, totally awesome.  Merry Christmas and Happy New Year
from
> one of Santa helpers to another.
> Tom Jasper
> Bend, OR (by way of northern WI)
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
> Claire
> Sent: Thursday, December 20, 2007 10:59 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Off Topic: NO SANTA CLAUS 
> 
>  
> Sorry if you are not Christian. You can delete, but I think Santa just
> has lots of different names around the world. Everyone - remember what
> you have that never cost anything. Merry Christmas greetings and warm
> wishes to all.
>  
> Claire Ingles
> lifelong Santa's helper
> 
>  
> 
> 					  I remember my first Christmas
> adventure with Grandma. 
> 					I was just a kid. 
> 					
> 					I remember tearing across town
> on my bike to visit her on the day my big sister dropped the bomb: 
> 					
> 					"There is no Santa Claus," she
> jeered. "Even dummies know that!" 
> 					
> 					My Grandma was not the gushy
> kind, never had been. 
> 					
> 					I fled to her that day because I
> knew she would be straight with me. I knew Grandma always told the
> truth, and I knew that the truth always went down a whole lot easier
> when swallowed with one of her worl d-famous cinnamon buns. 
> 					
> 					I knew they were world-famous,
> because Grandma said so. 
> 					It had to be true. 
> 					Grandma was home, and the buns
> were still warm. 
> 					
> 					Between bites, I told her
> everything. She was ready for me. 
> 					"No Santa Claus! !" she snorted.
> "Ridiculous! 
> 					" Don't believe it. That rumor
> has been going around for years, and it makes me mad, plain mad.' 
> 					
> 					Now, put on your coat, and let's
> go." 
> 					"Go? Go where, Grandma?" I
> asked. 
> 					I hadn't even finished my second
> world-famous, cinnamon bun. 
> 					
> 					"Where" turned out to be Kerby's
> General Store, the one store in town that had a little bit of just
about
> everything. As we walked through its doors, Grandma handed me ten
> dollars. That was a bundle in those days. 
> 					
> 					"Take this money," she said,
> "and buy something for someone who needs it. I'll wait for you in the
> car." 
> 					
> 					Then she turned and walked out
> of Kerby's. I was only eight years old. I'd often gone shopping with
my
> mother, but never had I shopped for anything all by myself. 
> 					
> 					The store seemed big and
> crowded, full of people scrambling to finish their Christmas shopping.
> For a few moments I just stood there, confused, clutching that
> ten-dollar bill, wondering what to buy, and who on earth to buy it
for. 
> 					
> 					I thought of everybody I knew:
> my family, my friends, my neighbors, the kids at school, the people
who
> went to my church. 
> 					
> 					I was just about thought out,
> when I suddenly thought of Bobby Decker. He was a kid with bad breath
> and messy hair, and he sat right behind me in Mrs. Pollock's grade-two
> class. Bobby Decker didn't have a coat. 
> 					
> 					I knew that because he never
> went out for recess during the winter. His mother always wrote a note,
> telling the teacher that he had a cough, but all we kids knew that
Bobby
> Decker didn't have a cough, and he didn't have a coat. 
> 					
> 					I fingered the ten-dollar bill
> with growing excitement. I would buy Bobby Decker a coat! I settled
on>  a
> red corduroy one that had a hood to it. It looked real warm, and he
> would like that. 
> 					
> 					"Is this a Christmas present for
> someone?" the lady behind the counter asked kindly, as I laid my ten
> dollars down. 
> 					
> 					"Yes," I replied shyly. "It's
> ... for Bobby." 
> 					
> 					The nice lady smiled at me. I
> didn't get any change, but she put the coat in a bag and wished me a
> Merry Christmas. 
> 					
> 					That evening, Grandma helped me
> wrap the coat in Christmas paper and ribbons (a little tag fell out of
> the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby,
From
> Santa Claus", on a tag-- Grandma said that Santa always insisted on
> secrecy. 
> 					
> 					Then she drove me over to Bobby
> Decker's house, explaining as we went that I was now and forever
> officially one of Santa's helpers. 
> 					
> 					Grandma parked down the street
> from Bobby's house, and she and I crept noiselessly and hid in the
> bushes by his front walk. Then Gr andma gave me a nudge. "All right,
> Santa Claus," she whispered, "get going." 
> 					I took a deep breath, dashed for
> his front door, and threw the present down on his step. I pounded his
> door and flew back to the safety of the bushes and Grandma. 
> 					
> 					Together we waited breathlessly
> in the darkness for the front door to open. Finally it did, and there
> stood Bobby. Fifty years haven't dimmed the thrill of those moments
> spent shivering beside my Grandma, in Bobby Decker's bushes. 
> 					
> 					That night, I realized that
> those awful rumors about Santa Claus were just what Grandma said they
> were: "Ridiculous". Santa was alive and well, and we were on his team.

> 					
> 					I still have the Bible, with the
> tag tucked inside: $19.95. 
> 					
> 	
> -------------------------------------------------------------------- 
> 					He who has no Christmas in his
> heart will never find Christmas under a tree. 
> 					
> 					Have a wonderful holiday season.
> Merry Christmas and a Happy New Year!! 
> 					
> 					
> 					
> 					
> 					
> 					
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


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------------------------------

Message: 10
Date: Thu, 20 Dec 2007 15:52:58 -0500
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: [Histonet] Tissue Tek anti-roll device
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <01MP4ABAC5UE0012GH <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=US-ASCII

Hi Guys, 
I am reaching for straws, but I need to locate an anti-roll device for
an
older model Lab Tek, Tissue Tek cryostat.  Mine finally was destroyed
after
ohhhhh, say 25 years.  Need to replace it or rebuild it.  Any help would
be
appreciated.
Thanks
Shirley Powell, HT(ASCP)HTL, QIHC
Technical Director Histopathology
Mercer University School of Medicine
1550 College Street
Macon, GA 31207
478-301-2374




------------------------------

Message: 11
Date: Thu, 20 Dec 2007 15:57:17 -0500
From: Phil McArdle <PMcArdle <@t> ebsciences.com>
Subject: Re: [Histonet] MICROWAVES
To: Janice Mitchell <MITCHELLJA <@t> email.chop.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <476AD72D.1080302 <@t> ebsciences.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi:

This response is from a microwave vendor, so you've been forewarned! :-)

That said, it seems analogous ISO-9000 certification: each lab is 
allowed to make its own determination as to the meaning of 
"periodically;" the procedure should be documented and assigned AND (and

here's the key part) adhered to and logged! I've seen some labs with 
very nicely thought out and documented procedures, who got written up 
because they didn't follow their own procedures.

When CAP began their microwave leakage measurement requirement, it 
specified "at least" yearly, and EBS recommended quarterly. (Arbitrary? 
Maybe.) As for reproducibility, I feel that yearly is not enough, daily 
or weekly is probably excessive, but depending upon your lab's volume, 
monthly or quarterly should be sufficient, unless anything unusual is 
noted (blown fuse, etc.).

Hope this helps!

Phil McArdle
-- 
Phil McArdle
Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel:  800.992.9037 x 341
Mobile: 860.597.6796
Fax: 860.653.0422

pmcardle <@t> ebsciences.com
www.ebsciences.com

I skate to where the puck is going to be, not to where it's been.
- Wayne Gretsky

You must be the change you want to see in the world.
- Mahatma Gandhi

NOTE: This message, together with any attachments, is intended only for 
the use of the individual or entity to which it is addressed and may 
contain information that is legally privileged, confidential and exempt 
from disclosure. If you are not the intended recipient, however, there's

not a lot I can do about it, and it was probably my mistake anyway. So 
please do the right thing and make this e-mail go away. Thank you.


Janice Mitchell wrote:
 > Cap requests "microwave devices be periodically monitored for
 > reproducibility" what is considered periodically?
 >                       Thanks, Janice
 >
 > _______________________________________________
 > Histonet mailing list
 > Histonet <@t> lists.utsouthwestern.edu
 > http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 12
Date: Thu, 20 Dec 2007 16:01:22 -0500
From: JGREWE <@t> OhioHealth.com
Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the
	office .
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF9E48DBAF.7AB1997C-ON852573B7.00737BA1-852573B7.00737BA1 <@t> ohiohealth.co
m>
	
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  12/20/2007 and will not return
until
12/24/2007.

I will return Tuesday November 13 at 11 PM and will respond to your
message
when I return. Thanks, Jackie




------------------------------

Message: 13
Date: Thu, 20 Dec 2007 13:09:33 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Tissue Tek anti-roll device
To: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF0C92718D.F519F096-ON882573B7.00742DEB-882573B7.007454CD <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Try Sakura.  That is where we bought ours, just a few of years ago.


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu



Shirley Powell <POWELL_SA <@t> Mercer.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/20/2007 12:52 PM

To
histonet <@t> lists.utsouthwestern.edu
cc

Subject
[Histonet] Tissue Tek anti-roll device






Hi Guys, 
I am reaching for straws, but I need to locate an anti-roll device for
an
older model Lab Tek, Tissue Tek cryostat.  Mine finally was destroyed 
after
ohhhhh, say 25 years.  Need to replace it or rebuild it.  Any help would

be
appreciated.
Thanks
Shirley Powell, HT(ASCP)HTL, QIHC
Technical Director Histopathology
Mercer University School of Medicine
1550 College Street
Macon, GA 31207
478-301-2374


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 14
Date: Thu, 20 Dec 2007 16:24:32 -0500
From: mtitford <@t> aol.com
Subject: [Histonet] Christmas Greetings, and thanks to...
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CA1165D8975232-D9C-6672 <@t> WEBMAIL-MB20.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

I would like to join in wishing everyone a happy Christmas, this time
from the "Heart of Dixie", ?and to thank Dr Margraf and others?for
hosting the "Histonet". Thanks also to all the subscribers whose
questions and answers help keep me informed.?Life is so interesting!

Mike Titford
USA Pathology
Mobile AL USA
________________________________________________________________________
More new features than ever.  Check out the new AOL Mail ! -
http://webmail.aol.com


------------------------------

Message: 15
Date: Thu, 20 Dec 2007 16:27:38 -0500
From: "Godfrey Guerzon" <Gguerzon <@t> lifebridgehealth.org>
Subject: [Histonet] IHC for VIAS
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: <histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID: <s76a9805.012 <@t> lifebridgehealth.org>
Content-Type: text/plain; charset=US-ASCII

We are having problem with tissue falling off the slides when we run the
breast panel on the Ventana XT for examination with the VIAS instrument.
 The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
place them in 45 degree oven overnight and they still fall of.

Any suggestions?

Thanks.
          

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Maryland.  Further disclosure of medical information contained 
herein is prohibited. 
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------------------------------

Message: 16
Date: Thu, 20 Dec 2007 16:46:07 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: SPAM-LOW:  [Histonet] IHC for VIAS
To: "'Godfrey Guerzon'" <Gguerzon <@t> lifebridgehealth.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<mailman.396.1198191826.19513.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

The first question that Ventana will ask is what brand of slides are you
using? 
If you are using the "correct slides" do you use any adhesive in your
water
bath? 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Godfrey
Guerzon
Sent: Thursday, December 20, 2007 4:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] IHC for VIAS

We are having problem with tissue falling off the slides when we run the
breast panel on the Ventana XT for examination with the VIAS instrument.
 The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
place them in 45 degree oven overnight and they still fall of.

Any suggestions?

Thanks.
          

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AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE 
ADDRESSEE LISTED ABOVE.
This record has been disclosed in accordance with Subtitle 3 of 
Title 4 of the Health-General Article of the Annotated Code of 
Maryland.  Further disclosure of medical information contained 
herein is prohibited. 
If you are neither the intended recipient nor the individual
responsible for delivering this message to the intended 
recipient, you are hereby notified that any disclosure of 
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------------------------------

Message: 17
Date: Thu, 20 Dec 2007 16:55:34 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: [Histonet] MITF
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.397.1198191826.19513.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have a good MITF protocol for the Ventana Benchmark XT?
Thanks.

 

 

Douglas D. Deltour HT(ASCP)

Histology Manager

Professional Pathology Services, PC

One Science Court

Suite 200

Columbia, SC 29203

Office (803)252-1913

Fax (803)254-3262

Doug <@t> ppspath.com 

*****************************************************

PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

 



------------------------------

Message: 18
Date: Thu, 20 Dec 2007 16:02:24 -0600
From: Kathy Abels <Kathy.Abels <@t> sial.com>
Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF0D7F6A84.E937DB11-ON862573B7.007911C7-862573B7.007911C7 <@t> sial.com>
Content-Type: text/plain;	charset="US-ASCII"


I will be out of the office starting  12/19/2007 and will not return
until
01/02/2008.

I will respond to your message when I return. For urgent matters, Please
contact Sherry Chappell or Leigh Gaskill.


This message and any files transmitted with it are the property of
Sigma-Aldrich Corporation, are confidential, and are intended
solely for the use of the person or entity to whom this e-mail is
addressed. If you are not one of the named recipient(s) or
otherwise have reason to believe that you have received this
message in error, please contact the sender and delete this message
immediately from your computer. Any other use, retention,
dissemination, forwarding, printing, or copying of this e-mail is
strictly prohibited.          



------------------------------

Message: 19
Date: Thu, 20 Dec 2007 14:11:40 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] IHC for VIAS
To: Godfrey Guerzon <Gguerzon <@t> lifebridgehealth.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C390289C.1253F%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"

I would recommend some time in a 60-65 degree oven even if you do still
want
to leave them in a 37-45 degree oven overnight.  That's just off the top
of
my head - busy day here.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> We are having problem with tissue falling off the slides when we run
the
> breast panel on the Ventana XT for examination with the VIAS
instrument.
> The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
> place them in 45 degree oven overnight and they still fall of.
> 
> Any suggestions?
> 
> Thanks.
>         
> 
> -----------------------------------------------------------------
> THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED
> AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE
> ADDRESSEE LISTED ABOVE.
> This record has been disclosed in accordance with Subtitle 3 of
> Title 4 of the Health-General Article of the Annotated Code of
> Maryland.  Further disclosure of medical information contained
> herein is prohibited.
> If you are neither the intended recipient nor the individual
> responsible for delivering this message to the intended
> recipient, you are hereby notified that any disclosure of
> patient information is strictly prohibited.
> If you have received this email in error, immediately notify us
> by telephone or return email.
> -----------------------------------------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------------------------------------------------
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This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any 
unauthorized review, use, disclosure or distribution is prohibited. If 
you are not the intended recipient, please contact the sender by e-mail 
and destroy all copies of the original message, or you may call
PhenoPath 
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.




------------------------------

Message: 20
Date: Thu, 20 Dec 2007 17:18:26 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] IgA-FITC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	
<979FF5962E234F45B06CF0DB7C1AABB214284CE9 <@t> chi2k3ms01.columbuschildrens.n
et>
	
Content-Type: text/plain;	charset="us-ascii"

Here's a new one for me, but I'm sure someone out there has been
approached about this (at least I hope so!)

Has anyone attempted to demonstrate IgA in human breast milk for
fluorescence photomicroscopy?

Preliminary attempts light up like a Christmas tree.................

Thanks and Merry Christmas
Ronnie


Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston <@t> NationwideChildrens.org
Columbus Children's Hospital is now Nationwide Children's Hospital
www.NationwideChildrens.org <http://www.nationwidechildrens.org/> 




------------------------------

Message: 21
Date: Thu, 20 Dec 2007 17:22:52 -0500
From: "richardkuzma <@t> michdiag.com" <richardkuzma <@t> michdiag.com>
Subject: [Histonet] Off topic:  Free samples of chemiluminescent
	substrates
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1928.1198189372 <@t> michdiag.com>
Content-Type: text/plain; charset="us-ascii"


   Michigan  Diagnostics  Is  offering  free  evaluation  samples  to
the
   research  co   number, so we don   Luminol  based HRP substrate and
dioxetane based AP substrate are what
   were    We  do  have  other  substrates for sale, including chemilumi
substrates  for  the detection of Beta-Glucosidase, Beta-Galactosidase
   and  Be   www.michdiag.c   Sorry  if  you  think this is too off
topic. Please don't flame me too
   badly.   Best Regards,
   Richard Kuzma
   Production Manager
   Michigan<   Royal Oak, MI. 48073 USA
   richardkuzma <@t> michdiag.com
   www.michdiag.com
   (248)435-4472
   (248)435-4537
   

------------------------------

Message: 22
Date: Thu, 20 Dec 2007 18:02:22 -0500
From: "Godfrey Guerzon" <Gguerzon <@t> lifebridgehealth.org>
Subject: Re: [Histonet] IHC for VIAS
To: <histonet <@t> lists.utsouthwestern.edu>,	"Patti Loykasek"
	<ploykasek <@t> phenopath.com>
Message-ID: <s76aae35.024 <@t> lifebridgehealth.org>
Content-Type: text/plain; charset=US-ASCII

Thanks Patti,

We have tried this method of 30 minutes in a 60degree oven and
overnight at 37-40 degree oven - it gives us better results but still
part of the tissue is falling off.

Thanks.

Godfrey

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 12/20/2007 5:11 PM >>>
I would recommend some time in a 60-65 degree oven even if you do still
want
to leave them in a 37-45 degree oven overnight.  That's just off the
top of
my head - busy day here.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> We are having problem with tissue falling off the slides when we run
the
> breast panel on the Ventana XT for examination with the VIAS
instrument.
> The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides
and
> place them in 45 degree oven overnight and they still fall of.
> 
> Any suggestions?
> 
> Thanks.
>         
> 
> -----------------------------------------------------------------
> THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED
> AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE
> ADDRESSEE LISTED ABOVE.
> This record has been disclosed in accordance with Subtitle 3 of
> Title 4 of the Health-General Article of the Annotated Code of
> Maryland.  Further disclosure of medical information contained
> herein is prohibited.
> If you are neither the intended recipient nor the individual
> responsible for delivering this message to the intended
> recipient, you are hereby notified that any disclosure of
> patient information is strictly prohibited.
> If you have received this email in error, immediately notify us
> by telephone or return email.
> -----------------------------------------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any 
unauthorized review, use, disclosure or distribution is prohibited. If

you are not the intended recipient, please contact the sender by e-mail

and destroy all copies of the original message, or you may call
PhenoPath 
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.


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AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE 
ADDRESSEE LISTED ABOVE.
This record has been disclosed in accordance with Subtitle 3 of 
Title 4 of the Health-General Article of the Annotated Code of 
Maryland.  Further disclosure of medical information contained 
herein is prohibited. 
If you are neither the intended recipient nor the individual
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------------------------------

Message: 23
Date: Thu, 20 Dec 2007 17:09:28 -0600
From: Annette Hall <annette_hall <@t> pa-ucl.com>
Subject: RE: [Histonet] IHC for VIAS
To: 'Patti Loykasek' <ploykasek <@t> phenopath.com>, Godfrey Guerzon
	<Gguerzon <@t> lifebridgehealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<71320B4EBC7C15419563EAFBFCD924651C7BD48A33 <@t> hades.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"

We had the same problem with our XT. We now put the slides at 60 degrees
for 1-2 hours. It's not perfect but we retain most of the tissue.

Annette J Hall, MT
Histo Supervisor
United Clinical Labs
Dubuque, Ia

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Thursday, December 20, 2007 4:12 PM
To: Godfrey Guerzon; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC for VIAS

I would recommend some time in a 60-65 degree oven even if you do still
want
to leave them in a 37-45 degree oven overnight.  That's just off the top
of
my head - busy day here.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> We are having problem with tissue falling off the slides when we run
the
> breast panel on the Ventana XT for examination with the VIAS
instrument.
> The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides and
> place them in 45 degree oven overnight and they still fall of.
>
> Any suggestions?
>
> Thanks.
>
>
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