[Histonet] RE: Histonet Digest, Vol 51, Issue 40

Thu Feb 28 08:18:00 CST 2008

Kappa/lambda CISH geniejacobs)
We are starting up Kappa lambda cish and would like any info on protocol and vendors  Does anyone have any experience using the Histosonda probe made by CENBIMO? thanks

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2008 12:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 51, Issue 40

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Today's Topics:

   1. FISH cytology fixatives (Kim Merriam)
   2. Re: FISH cytology fixatives (godsgalnow <@t> aol.com)
   3. Re: Fixing crab blood smear (Rene J Buesa)
   4. RE: Night differential (Cheri Miller)
   5. goldners trichrome (Simoskevitz <@t> Osteotech.com)
   6. Lyme Disease IHC/PCR (histosprv06 <@t> aol.com)
   7. Histology Supervisor Position NY (rmweber113 <@t> comcast.net)
   8. reference point slides. (Garry Ashton)
   9. reference point slides. (Garry Ashton)
  10. Dull eosin? (Martin, Erin)
  11. RE: Dull eosin? (Mike Pence)
  12. RE: Dull eosin? (Bartlett, Jeanine (CDC/CCID/NCZVED))
  13. FW: Region III Hosted by Georgia Society for	Histotechnology
      (Shirley Powell)

----------------------------------------------------------------------

Message: 1
Date: Wed, 27 Feb 2008 04:42:25 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] FISH cytology fixatives
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <668890.34731.qm <@t> web50312.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone,

  I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run).

  Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1).  The DAKO probe I am using calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure.  Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be?

  Thanks in advance,
  Kim

Kim Merriam, MA, HT(ASCP)
Cambridge, MA

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Message: 2
Date: Wed, 27 Feb 2008 08:24:11 -0500
From: godsgalnow <@t> aol.com
Subject: Re: [Histonet] FISH cytology fixatives
To: kmerriam2003 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CA475B2C9387AD-804-4E7 <@t> FWM-M08.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

In Cytological preparation, one would use an acetic acid in the fixative to lyse the RBC's, which could ultimaetly interfere with the diagnosis.? Journal articles are great to help you research and give you a starting point for what it is you want to do, that is where I started.? But when you start doing the procedure, or playing with until you get it to work, you always need to start with the suggestions of the manufacturer of the probe.? That is how they validated it and it is known to work under those conditions.? At that point you can play with fixatives, if you need to.

A lot of FISH testing requires post fixation anyway.? For, instance, the tissue comes in formalin (or whatever), but after you de-wax the slides you have to begin the pre-treatment, an most of the time there is a step in there that involves placing the slides in formalin (or whatever).

Roxanne

-----Original Message-----
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, 27 Feb 2008 7:42 am
Subject: [Histonet] FISH cytology fixatives

Hi Everyone,

  I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run).

  Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1).  The DAKO probe I am using calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure.  Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be?

  Thanks in advance,
  Kim

Kim Merriam, MA, HT(ASCP)
Cambridge, MA

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Message: 3
Date: Wed, 27 Feb 2008 05:37:27 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Fixing crab blood smear
To: "Cheah, Lit Chien" <lcheah <@t> agric.wa.gov.au>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <927292.99610.qm <@t> web61223.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Your procedure is standard, even for crab or spiny lobster for that matter (even when it really is hemolymph). They should be OK
  Do you know Dr. Phillips (working with spiny lobsters? I have lost contact with him since many years ago).
René J. Buesa

"Cheah, Lit Chien" <lcheah <@t> agric.wa.gov.au> wrote:
  Dear All, 

I have been ask is there a batter way to fix crab blood smear before the slides travel 800 kilometers for us to do Giemsa Stain, appart from the normal air dry then fix in methanol and shipment.

Big Thanks in advance from the largest State in Australia!

Mr. Lit Chien CHEAH
Histopathology Supervisor
BSC (Medical Science)

Animal Health Laboratories
3 Baron-Hay Court
South Perth, 6155 WA.

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Message: 4
Date: Wed, 27 Feb 2008 08:22:25 -0600
From: "Cheri Miller" <cmiller <@t> physlab.com>
Subject: RE: [Histonet] Night differential
To: "'Cindy DuBois'" <integrated.histo <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001501c8794c$2cd6f720$3d02a8c0 <@t> plab.local>
Content-Type: text/plain;	charset="us-ascii"

Where I used to work it was 15% of your hourly wage for any hours worked from 1100pm to 6am

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cindy DuBois
Sent: Tuesday, February 26, 2008 10:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Night differential

Are there any techs who get a night differential?  If so, how much?
We currently come to work @ 3:30 am.  We are now being asked to come in at 1:30.  I would like to ask for a night differential, but need to know how much to ask for.

Thanks,
Cindy
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Message: 5
Date: Wed, 27 Feb 2008 10:19:52 -0500
From: Simoskevitz <@t> Osteotech.com
Subject: [Histonet] goldners trichrome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF01CC8551.9C921F13-ON852573FC.0054023B-852573FC.00547602 <@t> Osteotech.com>

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Barbara,

I was asked to do a Goldners Trichrome in Methymethacylate embedded bone .
Do you have a good protocol for this or do you know where I could find one.

Thank you for any help you can give me.

Ricki Simoskevitz
Osteotech Inc.
Eatontown, NJ 07724
(732) 542-2800 X6328

------------------------------

Message: 6
Date: Wed, 27 Feb 2008 10:29:35 -0500
From: histosprv06 <@t> aol.com
Subject: [Histonet] Lyme Disease IHC/PCR
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CA476CB1044DEB-80C-AE3 <@t> webmail-da16.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Good Morning everyone, no better place to come for advice than the histonet! Does anyone know of a facility where?we can send a processed skin block for Borrelia Burgdorferi via IHC and PCR as well as Erlichiosis via PCR? We are in Florida and are not having much luck finding?a place?that offers this testing.?

We would greatly appreciate any knowledge you can provide.

Thanks in advance.

?
Kari Zajic, HT

------------------------------

Message: 7
Date: Wed, 27 Feb 2008 16:36:07 +0000
From: rmweber113 <@t> comcast.net
Subject: [Histonet] Histology Supervisor Position NY
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<022720081636.3418.47C591770005560100000D5A2215575114CCCECE9D0A0D0A99039D <@t> comcast.net>

Content-Type: text/plain

I have a position for a Histology Supervisor  available at an established GI group located in New City, NY, Rockland County.  Candidate should have the knowledge to set up and maintain a state of the art facility.  Knowledge of grossing, processing and routine histology of gastric biopsies required.  $30.00/hr plus benefits.
Qualified candidates can email me at rmweber113 <@t> comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for  at least 90 days.

Marilynn Weber H.T. (ASCP) QIHC
Twincrest

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Message: 8
Date: Wed, 27 Feb 2008 16:39:18 -0000
From: "Garry Ashton" <GAshton <@t> picr.man.ac.uk>
Subject: [Histonet] reference point slides.
To: <histonet <@t> lists.utsouthwestern.edu>,	"histonet"
	<Histonet <@t> pathology.swmed.edu>
Message-ID:
	<BAA35444B19AD940997ED02A6996AAE0045E7D8D <@t> sanmail.picr.man.ac.uk>
Content-Type: text/plain;	charset="us-ascii"

Hi everyone,
Has anybody used or had any experience of microscope slides with some type of reference point on them?
Thanks in advance.
Garry

PICR
UK

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Message: 9
Date: Wed, 27 Feb 2008 16:39:18 -0000
From: "Garry Ashton" <GAshton <@t> picr.man.ac.uk>
Subject: [Histonet] reference point slides.
To: <histonet <@t> lists.utsouthwestern.edu>,	"histonet"
	<Histonet <@t> pathology.swmed.edu>
Message-ID:
	<BAA35444B19AD940997ED02A6996AAE0045E7D8D <@t> sanmail.picr.man.ac.uk>
Content-Type: text/plain;	charset="us-ascii"

Hi everyone,
Has anybody used or had any experience of microscope slides with some type of reference point on them?
Thanks in advance.
Garry

PICR
UK

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This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.

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Message: 10
Date: Wed, 27 Feb 2008 09:06:53 -0800
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] Dull eosin?
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DA617A88F598DE4888173E76E7E0783D025E4397 <@t> EXVS07.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1

Hi everyone, 

I am having a problem with my eosin in the H&E.  My pathologists say that it is dull and the RBCs are too red rather than orange.  Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer.  Any ideas?

Thank in advance!

Erin Martin

------------------------------

Message: 11
Date: Wed, 27 Feb 2008 11:13:35 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Dull eosin?
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>,	"histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A373E <@t> IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

What eosin are you using?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, February 27, 2008 11:07 AM
To: histonet
Subject: [Histonet] Dull eosin?

Hi everyone, 

I am having a problem with my eosin in the H&E.  My pathologists say that it is dull and the RBCs are too red rather than orange.
Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer.  Any ideas?

Thank in advance!

Erin Martin

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Message: 12
Date: Wed, 27 Feb 2008 12:22:28 -0500
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> CDC.GOV>
Subject: RE: [Histonet] Dull eosin?
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>,	histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<34BB307EFC9A65429BBB49E330675F72045E25FA <@t> LTA3VS003.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii

I assume you are using the Richard Allan eosin Y alcoholic? 

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, February 27, 2008 12:07 PM
To: histonet
Subject: [Histonet] Dull eosin?

Hi everyone, 

I am having a problem with my eosin in the H&E.  My pathologists say
that it is dull and the RBCs are too red rather than orange.
Interestingly, when I take out the hematoxylin (Richard Allen Harris),
acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing)
the eosin is nice and bright. Our tap water for the rinses is pH 6.8,
and we use a Sakura Prisma stainer.  Any ideas?

Thank in advance!

Erin Martin

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 13
Date: Wed, 27 Feb 2008 12:25:09 -0500
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: [Histonet] FW: Region III Hosted by Georgia Society for
	Histotechnology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <01MRSH6CO2WQ001E0L <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii

I sent this out yesterday, but I never received it myself so this is just to
make sure you guys get it.

-----Original Message-----
From: Shirley Powell [mailto:powell_sa <@t> mercer.edu] 
Sent: Tuesday, February 26, 2008 4:48 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: Region III Hosted by Georgia Society for Histotechnology

Hi Guys, 

We sent out over 1000 programs for the Region III meeting to be held in
Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel.  But
there may be some members that we missed and feel it necessary to post a
reminder for all who wish to attend our meeting.  

The deadline for the hotel reservations for the discounted rate is March
3rd.  That rate is $129 single, double, triple or quad.  The phone number to
the Westin is 1-404-659-1400 and please state that you are attending the
GSH/Region III meeting.  

The revised program can be downloaded from our website at
www.histosearch.com/gsh.  Click on the symposium link to get a PDF file of
the program.  Vendors have a link to the registration form to exhibit at the
meeting on the same page.  If you have questions Chris Coley, GSH Exhibit
Liaison, has contact information is on that form

If anyone has any questions please feel to contact me at this email address.

Come on down to Georgia and experience some warmer weather.  

Shirley Powell
GSH Secretary/Registrar

------------------------------

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End of Histonet Digest, Vol 51, Issue 40
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