[Histonet] FISH cytology fixatives

[Tony Henwood]

If you are looking for DNA translocations, 95% ethanol works well.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
Merriam
Sent: Wednesday, 27 February 2008 11:42 PM
To: Histonet
Subject: [Histonet] FISH cytology fixatives


Hi Everyone,
   
  I am working up some FISH staining protocols for cytology (cell
culture) slides (eventually to be OCT and FFPE tissues, but I need to
walk before I can run).
   
  Most of the journals that I have read recommend using a fixative
containing methanol and acetic acid (3:1).  The DAKO probe I am using
calls for the use of 3.7% fomaldehyde.  I am wondering why acetone (or
acetone/ethanol) is not the routine fixative for such a procedure.  Can
someone let me know why to choose one fixative over another and what the
merits of methanol/glacial acetic acid would be?
   
  Thanks in advance,
  Kim


Kim Merriam, MA, HT(ASCP)
Cambridge, MA
       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************