[Histonet] Basic Fuchsin vs. Pararosaniline

[Geoff McAuliffe]

Hi Mareike:

    It depends on how much pararosanaline is in your basic fuchsin. 
According to John Kiernan modern samples of BF are almost entirely 
pararosanaline or rosanaline. Older samples may also contain new fuchsin 
and/or magenta II. If the dye is certified by the Biological Stain 
Commission you could contact them and find out what is in your batch. If 
it is not certified all you can do it try it or buy a new supply. Again 
we see the advantage of using certified dyes.

Geoff

Mareike Heimann wrote:
> Dear all, 
>
> I would like to try using the Leder stain for mast cells. Before I buy pararosaniline, which is not available in our lab, I wanted to ask if it is possible to use Basic Fuchsin (which we do have) instead?
>
> Thanks in advance for your advice, 
> Mareike Heimann
> University of Iceland
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>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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