[Histonet] Speaking of Fluoro Jade

[John Kiernan]

Perhaps it need not be too expensive. According to US Patent 6,229,024 (Schmued 2001) Fluoro-Jade is a sodium salt made by suspending 1 g of 5(6)-carboxyfluorescein in 10 ml water, adding NaOH until  the pH of the resulting solution is between 7 and 8, and then removing the water by lyophilization or by evaporation in a desiccator to obtain the crystalline salt. You can buy 5(6)-carboxyfluorescein for $37 a gram (e.g. Sigma catalogue no. 21877). Solutions should be stable if protected from light and growth of moulds. 
 
Sodium carboxyfluorescein is an anionic xanthene dye, as is eosin Y, which also stains dead cells strongly and is fluorescent.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Amos Brooks <amosbrooks <@t> gmail.com>
Date: Tuesday, December 16, 2008 18:13
Subject: [Histonet] Speaking of Fluoro Jade
To: tgenade <@t> gmail.com, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

> Hi,
>    Speaking of Fluoro Jade: I don't do this often and 
> after having done the
> stain recently I was left with a lot of extra Fluoro Jade stock 
> solution.The data sheet says this is only good for a week or so 
> making it a dang
> expensive test! For non-diagnostic use of course, could the rest be
> aliquated and frozen (-80 C) to use later?
> 
> Thanks,
> Amos
> 
> 
> Date: Tue, 16 Dec 2008 12:24:38 +0200
> From: "Tyrone Genade" <tgenade <@t> gmail.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 61, Issue 25
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>        
> <a801af370812160224mdf3de28o1a565112e6c93f18 <@t> mail.gmail.com>Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi all,
> 
> <SNIP>
> 
> Otherwise, you may want to look into FlouroJadeB. If all you are after
> is visualizing degenerating axons, there are easier ways of going
> about ot than silver staining.
> 
> Kind regards
> --
> Tyrone Genade
> http://tgenade.freeshell.org
> email: tgenade <@t> freeshell.org
> tel: +27-84-632-1925 (c)
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