[Histonet] Isopentane

[Tony Henwood]

Depends on how you freeze.
When we freeze tissue in liquid nitrogen, the tissue is shaken, ensuring
that the gas layer is broken up.
And in so doing we have not seen evidence of ice crystal formation.

I can send you a reprint of a paper that describes the effect of storage
of brain tumours in saline prior to freezing (causing much distortion of
the tumour) verses receiving the samples fresh on damp gauze or card.
The pics show the evidence.

Henwood, A., (2007) "Adverse effect of saline on brain intraoperative
(frozen section) Histology" J Histotechnol 30(3):193

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: Markus F. Meyenhofer [mailto:micro <@t> superlink.net] 
Sent: Wednesday, 17 December 2008 1:47 PM
To: Tony Henwood; Dana Settembre; carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane


Isopentane is cooled by liquid N2!
To freeze tissue direct in LN2  is not the best way to freeze tissue due
to 
the Leidenfrost effect, creating an insulating gas layer between the
tissue 
and the liquid and delaying the speed of freezing = an increase in ice 
crystal formation.
Regards,
Markus F. Meyenhofer

----- Original Message ----- 
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
To: "Dana Settembre" <settembr <@t> umdnj.edu>; "carmen loiselle" 
<carmen_loiselle <@t> hotmail.com>; "pathology education" 
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, December 16, 2008 6:19 PM
Subject: RE: [Histonet] Isopentane


Try liquid nitrogen

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Wednesday, 17 December 2008 5:27 AM
To: carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane


Hello Carmen,
Sorry I have no alternative.  I know most labs use that.
When I worked in a neuropathology lab the isopentane was kept in the
refrigerator - an explosion proof one. I believe that means there's no
lightbulb inside and no inner working of a lightbulb inside either.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> carmen loiselle <carmen_loiselle <@t> hotmail.com> 12/16/08 12:24 PM
>>>

Hello ,

I currently work in a neuromuscular lab and all muscle biopsies received
should be cut frozen, no formalin fixed tissues.  My concern though, is
to work with isopentane , a well known excellent heat conductor but also
it has the bad reputation of being very explosive. In fact , I've been
told that few years ago, a lab close by of where I work , did explode
because of this solution.  Is there , in your knowledge, any other
chemical we can use as replacement and that give as good results.

Any input will be greatly appreciated

Merry XMas to all and happy New Year
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