[Histonet] at a troubleshooting loss

[Michele Wich]

A couple questions for those who are troubleshooting savvy: Other than
inadequate fixation, water being introduced during processing, or too
much heat, what can cause fuzzy, uneven hematoxylin staining with poor
nuclear detail? 

And secondly, aside from formalin, are there any other common agents of
blackish pigment in H & E staining? (The artifact appears on the slides
both on and around the tissues.) 

Any feedback is GREATLY appreciated.

 


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