[Histonet] pH testing

[Hobbs, Carl]

I would be very grateful if John Kiernan had the time to respond to my question.

We all talk about pH  but, many of us have no idea how to make sure, for eg, that we are actually measuring pH values accurately.
How should we do that?
I use  TRIS buffer for my Immunostaining : I have a TRIS pH electrode.
Is this absolutely reqd?
I use VWR Std buffers to check my pH meter once a week: I check ph4,7 and 10.
Is this neccessary?
Respectfully,
Carl