[Histonet] perfusion question

[Geoff McAuliffe]

Does not matter. The only error I see people making is spending a lot of 
time on the washout step. You will never get all of the blood out so 
there is no reason to try. Given a 250 g rat, I would use 25-30 ml of 
washout instilled in 15-20 seconds, then switch over to fix.

Geoff


Neil Fournier wrote:
> Is there a rationale for using normal saline (0.9% (w/v) NaCl dissolved in dH2O) over 0.1 M PBS (pH 7.4) as a rinsing solution during intraventricular perfusion of a rat. Would one yield better results over the other?
>
> Also is there a raionale for why some people perfuse using PBS made only from monobasic and dibasic sodium phosphate (with 0.9% NaCl) vs. using PBS that also include KCl, sodium phosphate dibasic, NaCl, and potassium phosphate monobasic in the recipe.
>
> Thanks for the help
>
> Neil 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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