[Histonet] perfusion question

[Neil Fournier]

Is there a rationale for using normal saline (0.9% (w/v) NaCl dissolved in dH2O) over 0.1 M PBS (pH 7.4) as a rinsing solution during intraventricular perfusion of a rat. Would one yield better results over the other?

Also is there a raionale for why some people perfuse using PBS made only from monobasic and dibasic sodium phosphate (with 0.9% NaCl) vs. using PBS that also include KCl, sodium phosphate dibasic, NaCl, and potassium phosphate monobasic in the recipe.

Thanks for the help

Neil