[Histonet] RE: Histonet Digest, Vol 61, Issue 16
Martin, Jessica
jmartin <@t> lrgh.org
Wed Dec 10 14:45:29 CST 2008
Hi all,
Diane,
We also have the Thermo Consul coverslipper and we switched to Mercedes Medical coverslips some time ago. We are actually having much better luck with them and they are way less expensive. Contact Jake Smith at 1-800-359-8807. The order number that we use is r2450. He sent us samples and we liked them. I hope this helps. (No, I don't get any kick-backs for this!)
Jessica Martin HT, ASCP
x-3231
jmartin <@t> lrgh.org
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, December 10, 2008 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 61, Issue 16
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Today's Topics:
1. RE: thermometer calibration (Joyce Cline)
2. coverslip issues (Denise Crowley)
3. (no subject) (Louise Stillman)
4. Re: (no subject) (Peter Carroll)
5. Primary works with ABC but not IF (Patten, Nicole (NIH/NIAAA) [F])
6. Histology Workload Guidelines (Gagnon, Eric)
7. RE: Histology Workload Guidelines (Mike Pence)
8. RE: Histology Workload Guidelines (bhewlett <@t> cogeco.ca)
9. Re: Primary works with ABC but not IF (Kim Merriam)
10. Processing mouse seminal vesicles (Kathleen Roberts)
11. Re: Histology Workload Guidelines (Peggy Bisher)
12. Re: Processing mouse seminal vesicles (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Tue, 9 Dec 2008 13:15:09 -0500
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: RE: [Histonet] thermometer calibration
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C39E8705A78C4AFEB7E06CD706129160 <@t> wchsys.org>
Content-Type: text/plain; charset="us-ascii"
I have purchased a calibrated digital thermometer. I take the temps using
the calibrated thermometer and the regular thermometer. Compare the
difference and list it at the top of my Quality Control sheets for the
instruments. When the temps are taken everyday with the regular thermometer
they are listed on the daily record sheet. These temps should fall within
the designated temps. This worked for my last inspection.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margiotta,
Michele
Sent: Monday, December 08, 2008 9:28 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] thermometer calibration
Hi All,
Just wondering how everyone is doing their thermometer calibrations.
Inspection time is right around the corner so I want to be ready!
Thanks.
Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192
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Message: 2
Date: Tue, 9 Dec 2008 14:12:31 -0500
From: Denise Crowley <dencrowl <@t> MIT.EDU>
Subject: [Histonet] coverslip issues
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <E90A17A9-9086-4CD1-8FA2-86520BB4C578 <@t> mit.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Hi all,
I'm looking for some help in choosing a new vendor for coverslips for
the Thermo Consul coverslipper. We have been buying coverslips from
Thermo since we bought the instrument, and the last shipment (of 10
cases) is from a new supplier and is not working in the instrument.
The coverslip cannot be detected and the mountant is not dispensing.
I spoke with the technician (Diane is wonderful) and she walked me
through a diagnostic on the Consul and the instrument is not the
problem. Does anyone out there have a suggestion as to a coverslip
that they know works in the Consul? I have already tried the Vista
from VWR (which is okay, but not perfect) and am eagerly awaiting
requested samples. Thanks for your help.
Denise Crowley
Facility Manager Histology
David H. Koch Institute for Integrative Cancer Research
Massachusetts Institute of Technology
40 Ames St. E17-427
Cambridge MA 02139
617-258-8183
dencrowl <@t> mit.edu
------------------------------
Message: 3
Date: Tue, 9 Dec 2008 13:17:30 -0800 (PST)
From: Louise Stillman <stillmanlouise <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <29294.10789.qm <@t> web46305.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
unsubscribe? E-Mail address:? stillmanlouise <@t> yahoo.com
------------------------------
Message: 4
Date: Tue, 09 Dec 2008 16:30:21 -0500
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] (no subject)
To: Louise Stillman <stillmanlouise <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <493EE36D.8050005 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hey Louise,
See that link below, at the bottom of your email? The one that connects
you to the page where you can unsubscribe yourself?
Click it!
Just another friendly reminder that theres no Unsubscribe Fairy lurking
about this list making your unsubscription dreams come true... you can
actually do it yourself with merely a few clicks and a little typing!
Louise Stillman wrote:
> unsubscribe E-Mail address: stillmanlouise <@t> yahoo.com
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
------------------------------
Message: 5
Date: Wed, 10 Dec 2008 10:09:45 -0500
From: "Patten, Nicole (NIH/NIAAA) [F]" <pattennj <@t> mail.nih.gov>
Subject: [Histonet] Primary works with ABC but not IF
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FF65463314D83A43BCD51C03A134E4015D9C56 <@t> NIHCESMLBX.nih.gov>
Content-Type: text/plain; charset="US-ASCII"
Hello-
I am in a situation where my primary antibody works using the ABC
method, but I do not see staining with immunofluorescence. My IF
protocol works well with other antibodies so I do not know why it's not
working with this particular antibody. Does anybody have any
suggestions?
I have considered using an avidin-Alexa Fluor conjugate but I am not
sure if this is even possible (I am new to IHC) or how I would go about
it. Would I need to first biotinylate my primary?
Does anyone have any advice/protocols?
Thanks! Any help would be greatly appreciated.
Nicole Patten
Post-Baccalaureate Fellow/IRTA
NIAAA/National Institutes of Health
------------------------------
Message: 6
Date: Wed, 10 Dec 2008 10:10:34 -0500
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] Histology Workload Guidelines
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F93BD6329FC3AE4C8DB116B985FBC31327C3A810 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain; charset="iso-8859-1"
To add to the recent discussion about how many blocks can be embedded per hour, the College of Medical Laboratory Technologists of Ontario recently published MLT practice guidelines which may be of use. The practice guidelines are "intended to support, not replace, the exercise of professional judgment by medical laboratory technologists practising in histology...and are maintained...in consultation with CMLTO members and stakeholders." Check out quality assurance at:
http://cmlto.com
or more specifically:
http://cmlto.com/quality_assurance/MLT_practice_guidelines/learning/10_hsto_gdlne.pdf
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada
------------------------------
Message: 7
Date: Wed, 10 Dec 2008 09:33:04 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Histology Workload Guidelines
To: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A39DB <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
Why are times bases on 37.5 hour works per week?
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gagnon,
Eric
Sent: Wednesday, December 10, 2008 9:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histology Workload Guidelines
To add to the recent discussion about how many blocks can be embedded
per hour, the College of Medical Laboratory Technologists of Ontario
recently published MLT practice guidelines which may be of use. The
practice guidelines are "intended to support, not replace, the exercise
of professional judgment by medical laboratory technologists practising
in histology...and are maintained...in consultation with CMLTO members
and stakeholders." Check out quality assurance at:
http://cmlto.com
or more specifically:
http://cmlto.com/quality_assurance/MLT_practice_guidelines/learning/10_h
sto_gdlne.pdf
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 10 Dec 2008 11:03:04 -0500
From: bhewlett <@t> cogeco.ca
Subject: RE: [Histonet] Histology Workload Guidelines
To: "Mike Pence" <mpence <@t> grhs.net>, "Gagnon, Eric"
<gagnone <@t> KGH.KARI.NET>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <493fe838.182.25af.14976 <@t> cogeco.ca>
Because that is the length of time for a standard work week in this province.
Bryan
> Why are times bases on 37.5 hour works per week?
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gagnon,
> Eric
> Sent: Wednesday, December 10, 2008 9:11 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histology Workload Guidelines
>
>
> To add to the recent discussion about how many blocks can be embedded
> per hour, the College of Medical Laboratory Technologists of Ontario
> recently published MLT practice guidelines which may be of use. The
> practice guidelines are "intended to support, not replace, the exercise
> of professional judgment by medical laboratory technologists practising
> in histology...and are maintained...in consultation with CMLTO members
> and stakeholders." Check out quality assurance at:
>
> http://cmlto.com
>
> or more specifically:
>
> http://cmlto.com/quality_assurance/MLT_practice_guidelines/learning/10_h
> sto_gdlne.pdf
>
> Eric Gagnon MLT
>
> Histology Laboratory
>
> Kingston General Hospital,
>
> Kingston, Ontario, Canada
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 10 Dec 2008 08:05:40 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: Re: [Histonet] Primary works with ABC but not IF
To: "Patten, Nicole \(NIH/NIAAA\) \[F\]" <pattennj <@t> mail.nih.gov>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <858018.38695.qm <@t> web50301.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Nicole,
I don't know what kind of?secondary antibody you are using, but we have had very good luck with the Alexa-Fluor secondaries.??Often, when you go from an amplified?chromagenic method (such as ABC) to?a less-amplified staining method (such as with a?fluorescently-labeled secondary), the primary antibody needs to be retitrated.? Perhaps there is not?enough amplification with a directly labeled seconday; you may need to add another lay or do additional amplification, such as with tyramide - we are having very good luck with Invitrogen's?TSA?kits that contain AlexaFluor dyes.? The Perkin Elmer FITC-TSA kits also work well, but cost about 3X as much as Invitrogens.
If you send more information about your staining protocols, we could probably give you more specific advice.
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
________________________________
From: "Patten, Nicole (NIH/NIAAA) [F]" <pattennj <@t> mail.nih.gov>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 10, 2008 10:09:45 AM
Subject: [Histonet] Primary works with ABC but not IF
Hello-
I am in a situation where my primary antibody works using the ABC
method, but I do not see staining with immunofluorescence. My IF
protocol works well with other antibodies so I do not know why it's not
working with this particular antibody. Does anybody have any
suggestions?
I have considered using an avidin-Alexa Fluor conjugate but I am not
sure if this is even possible (I am new to IHC) or how I would go about
it. Would I need to first biotinylate my primary?
Does anyone have any advice/protocols?
Thanks! Any help would be greatly appreciated.
Nicole Patten
Post-Baccalaureate Fellow/IRTA
NIAAA/National Institutes of Health
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Wed, 10 Dec 2008 11:54:32 -0500
From: Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
Subject: [Histonet] Processing mouse seminal vesicles
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <493FF448.7020001 <@t> rci.rutgers.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Does anybody have a protocol for this? My last batch of these came out
VERY dry and crunchy when run with other tissues on my standard
protocol, which is as follows:
(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out
before processing.)
70%: 30 min
80%: 30 min
95%: 45 min
95%: 45 min
100%: 45 min
100%: 45 min
xylene: 45 min
xylene: 45 min
Paraffin: 30 min
Paraffin: 30min
Paraffin: 30 min
My other thought is that something is up with our VIP 5 processor, though no error messages are showing up. Any and all suggestions are most welcome.
Thanks in advance,
Kathleen Roberts
Rutgers University
------------------------------
Message: 11
Date: Wed, 10 Dec 2008 12:12:57 -0500
From: Peggy Bisher <mbisher <@t> Princeton.EDU>
Subject: Re: [Histonet] Histology Workload Guidelines
To: <bhewlett <@t> cogeco.ca>, Mike Pence <mpence <@t> grhs.net>, "Gagnon, Eric"
<gagnone <@t> KGH.KARI.NET>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C56562C9.ACB9%mbisher <@t> exchange.princeton.edu>
Content-Type: text/plain; charset="US-ASCII"
We (Princeton University) are also on a 37.5 hour week. That takes into
account 30 minutes for a lunch break.
Peggy
On 12/10/08 11:03 AM, "bhewlett <@t> cogeco.ca" <bhewlett <@t> cogeco.ca> wrote:
> Because that is the length of time for a standard work week in this province.
>
> Bryan
>
>
>
>> Why are times bases on 37.5 hour works per week?
>>
>> Mike
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gagnon,
>> Eric
>> Sent: Wednesday, December 10, 2008 9:11 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Histology Workload Guidelines
>>
>>
>> To add to the recent discussion about how many blocks can be embedded
>> per hour, the College of Medical Laboratory Technologists of Ontario
>> recently published MLT practice guidelines which may be of use. The
>> practice guidelines are "intended to support, not replace, the exercise
>> of professional judgment by medical laboratory technologists practising
>> in histology...and are maintained...in consultation with CMLTO members
>> and stakeholders." Check out quality assurance at:
>>
>> http://cmlto.com
>>
>> or more specifically:
>>
>> http://cmlto.com/quality_assurance/MLT_practice_guidelines/learning/10_h
>> sto_gdlne.pdf
>>
>> Eric Gagnon MLT
>>
>> Histology Laboratory
>>
>> Kingston General Hospital,
>>
>> Kingston, Ontario, Canada
>>
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 10 Dec 2008 09:32:08 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Processing mouse seminal vesicles
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>, Kathleen Roberts
<kgrobert <@t> rci.rutgers.edu>
Message-ID: <213720.77503.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Mouse tissues, any one, will dry out too much with ethanol and xylene.
I advise you to stop using ethanol and xylene and substitute BOTH with iso-propanol at the concentrations and times you are using now.
Instead of the first paraffin, use a mixture 1:1 of iso-propanol and paraffin, followed by the 2 paraffins.
Try it and you will notice the difference (and the saings).
Ren? J.
--- On Wed, 12/10/08, Kathleen Roberts <kgrobert <@t> rci.rutgers.edu> wrote:
From: Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
Subject: [Histonet] Processing mouse seminal vesicles
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, December 10, 2008, 11:54 AM
Does anybody have a protocol for this? My last batch of these came out VERY dry
and crunchy when run with other tissues on my standard protocol, which is as
follows:
(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before
processing.)
70%: 30 min
80%: 30 min
95%: 45 min
95%: 45 min
100%: 45 min
100%: 45 min
xylene: 45 min
xylene: 45 min
Paraffin: 30 min
Paraffin: 30min
Paraffin: 30 min
My other thought is that something is up with our VIP 5 processor, though no
error messages are showing up. Any and all suggestions are most welcome.
Thanks in advance,
Kathleen Roberts
Rutgers University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 61, Issue 16
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