[Histonet] Processing mouse seminal vesicles

Kathleen Roberts kgrobert <@t> rci.rutgers.edu
Wed Dec 10 10:54:32 CST 2008


Does anybody have a protocol for this?  My last batch of these came out 
VERY dry and crunchy when run with other tissues on my standard 
protocol, which is as follows:
(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out 
before processing.)

70%: 30 min
80%: 30 min
95%: 45 min
95%: 45 min
100%: 45 min
100%: 45 min
xylene: 45 min
xylene: 45 min
Paraffin: 30 min
Paraffin: 30min
Paraffin: 30 min

My other thought is that something is up with our VIP 5 processor, though no error messages are showing up.  Any and all suggestions are most welcome.

Thanks in advance,
Kathleen Roberts
Rutgers University





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