[Histonet] Re: NG2

[Masaaki Kitada]

As far as my experience, antigen retrieval with microwave treatment
destroyed the antigenicity of NG2, although I used rabbit NG2 antibody
given by Dr. Stallcup in immunostaining for cryosections so there should be
some differences from your condition. I don't know what's happening in
there.

Masaaki Kitada

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   Masaaki Kitada, M.D., Ph.D.

   Associate Professor
   Department of Stem Cell Biology and Histology
   Tohoku Univeristy Graduate School of Medicine
   2-1, Seiryo-machi, Aoba-ku
   Sendai, Miyagi 980-8575, Japan
   Phone: +81-22-717-8029, FAX: +81-22-717-8030
   e-mail: Masaaki.Kitada <@t> gmail.com

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At 9:45 AM +0000 08.12.5, Hobbs, Carl wrote:
>I use Chemicon's AB5320 anti NG2 ( 1/50-100) on mouse and rat pwax brain
>sections. I need  AR and use Citric acid pH6 in a microwaveable pressure
>cooker.Standard stABCpxDAB detection.
>Apart from the poor dilution factor, it's a very good reagent, imo.
>
>Carl Hobbs
>Histology Manager
>Wolfson Centre for Age-Related Diseases
>King's College London
>Tel.020 7848 6810
>
>
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