I use Chemicon's AB5320 anti NG2 ( 1/50-100) on mouse and rat pwax brain sections. I need AR and use Citric acid pH6 in a microwaveable pressure cooker.Standard stABCpxDAB detection. Apart from the poor dilution factor, it's a very good reagent, imo. Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases King's College London Tel.020 7848 6810