[Histonet] PSA-NCAM IHC on frozen section with BrdU

[tf]

Hi everyone, it seems that I lost the PSA-NCAM staining when sections are treated with citrate buffer (poring the membrane?)
Possibly the membrane was dissociated from membrane then>? or?

For co-staining with BrdU, I can only treat sections with HCl - the results sometimes are unstable~~

Anyone worked on this before?

2008-12-04 



tf 
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