[Histonet] GMA embedded section attachment to slides
ratliffjack <@t> hotmail.com
Wed Dec 3 20:12:29 CST 2008
Is it possible for you to provide more information as to the type of specimen you are working with and how thick you are cutting the sections?
Jack> Date: Mon, 1 Dec 2008 16:26:31 -0600> From: Herrick.James <@t> mayo.edu> To: histonet <@t> lists.utsouthwestern.edu> Subject: [Histonet] GMA embedded section attachment to slides> > Hi everyone,> > I have been trying to attach Glycol Methacrylate (GMA) embedded specimens to glass slides and have not been having much luck. So far I have tried gelatin, APES and Histostik. I am using a press and placing them in a 50° C oven for approx. 48 hours. The sections look as if they are attached, until I begin staining. I am pretty sure that the PEG is causing my problem, but have seen an article or two that have attached GMA sections to slides. Does anyone have a protocol that works well with GMA? If so, I would greatly appreciate any help I can get. Thank you much.> > Jim> > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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