[Histonet] Expiration date checks

[CLAIRE ANN KENTLER]

Hi All,

Whilst we're on the subject, we're currently trying to troubleshoot an
ELISA assay...  This may be a silly question, but does anyone know if
(or when) Sodium Bicarbonate loses its buffering capacity?  (I.e. does
it expire?)

Thanks,
Claire

************************************************************************
***************************************
Claire Kentler
Bachelor of Animal Science (Hons)
Postgraduate Student
Department of Agricultural Sciences
La Trobe University
Phone: 9479 1048
E-mail: cabramley <@t> students.latrobe.edu.au
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rande
Kline
Sent: Wednesday, 3 December 2008 2:18 AM
To: Weber, Susan (VHACLE)
Cc: histonet
Subject: Re: [Histonet] Expiration date checks

Patty,

Chemicals really don't last forever.  Changes take place everytime you
open the bottle and some just from age even unopened.  Salts used in
buffers are hygroscopic.  They may appear to look okay but they may not
be for your application.  How long you can use a chemical is dependent
on it's application.  I think everyone has experienced a solution not
working even though you have followed the correct procedure and a repeat
has the same outcome.

I have wrote a couple of articles a few years back that appeared in
Laboratory Medicine Q&A and Microscopy Today on expiration dating of
chemicals.  The article in Microscopy Today is a little more indepth. I
would not mind faxing you a copy.  You may want to dig into   more than
the
dot system.  I would also suggest disposing chemicals that you may not
know the actual age of, if they exist.


On Tue, Dec 2, 2008 at 8:48 AM, Weber, Susan (VHACLE)
<Susan.Weber2 <@t> va.gov>wrote:

> Color coding with sticker "dots" helps a bit. I designate a color for 
> each year (5 yrs/5 colors max) On the top of that years color I put a 
> date (usually 6/08 whatever) and make them easier to spot. (Pardon the

> pun!)You can find color dots in the office supply catalog, Office Max 
> (or similar) or the craft stores. Happy Holidays To All!
>
> Sue Weber
>  -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti 
> Loykasek
> Sent: Monday, December 01, 2008 6:49 PM
> To: histonet
> Subject: [Histonet] Expiration date checks
>
>  Hi all. I am hoping for some clever ideas on the best method to check

> for expired reagents. We all know that we shouldn't use them, I'm sure

> we all actively monitor this (right)! I must admit we occasionally 
> have expired reagents - a stain or buffer we haven't used in a while 
> is in a cupboard or fridge & it has expired. What do others do to 
> monitor & prevent this from happening? We have a weekly safety check 
> that spot checks a certain # of reagents for expiration, but we 
> probably have thousands of reagents & don't check every single bottle 
> every week. Thanks for the input.
>
> Patti Loykasek
>
>
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--
Rande Kline, HT (ASCP)
Histopathology Lab Concepts
P-609-744-0803
F-609-939-0270
randek <@t> histopathconcepts.com
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