AW: [Histonet] Question RE: HQIP Result

[Gudrun Lang]

Schaundra,
In the following publication Fox describes bubbles that occur in cultered
cells after 5-30 min after the addition of formaldehyd solution.
Fox Cecil H ua., Formaldehyd Fixation; J. of. Histochemistry and
Cytochemistry 33(8):845-853, 1985

So bubbles could be a sign of very short fixation.
Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Schaundra
Walton
Gesendet: Montag, 01. Dezember 2008 18:58
An: Histonet
Betreff: [Histonet] Question RE: HQIP Result 

We just got our HQIP results and they made a comment regarding the breast
tissue H&E slide we submitted.  Can someone please tell me what nuclear
bubbling artifact is and what causes it?  
 
Thanks!
Schaundra Walton BS, HTL (ASCP)
Histology Supervisor
Swedish American Hospital 
Rockford, IL


      
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