[Histonet] Substitute Hematoxylin Dyes

[Dana Settembre]

Hello Deanna,
I can only tell you about how Phoenix Blue worked as a counterstain
for immunos and it does not turn blue in the ammonia.
It remained a lilac color which was difficult with the DAB.
If the DAB staining was light on the patient case it was tough.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Snider, Vivian Deanna" <vsnider <@t> shrinenet.org> 08/14/08 11:00 AM
>>>
Dear Netters,

What are you substituting for Hematoxylin?  I have narrowed it down to
2
I want to research; The Tango Stain, from Anatech and Phoenix Blue
Nuclear Stain, ordered from Fisher.  Is anyone familiar with either of
these stains?  If so, what are the positive/negatives about each one?
Are the results as comparable to the actual Hematoxylin dye?  I have
searched the net for images of a completed stain, to no avail.  Any
information will be greatly appreciated .

 

Thanks in advance.

Deanna Leslie HT ASCP

Lead Histotechnician

Histology Research Laboratory

Shriners Hospital for Children

Cincinnati, OH 

 

 



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