[Histonet] Substitute Hematoxylin Dyes

Dana Settembre settembr <@t> umdnj.edu
Thu Aug 14 10:16:26 CDT 2008

Hello Deanna,
I can only tell you about how Phoenix Blue worked as a counterstain
for immunos and it does not turn blue in the ammonia.
It remained a lilac color which was difficult with the DAB.
If the DAB staining was light on the patient case it was tough.

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> "Snider, Vivian Deanna" <vsnider <@t> shrinenet.org> 08/14/08 11:00 AM
Dear Netters,

What are you substituting for Hematoxylin?  I have narrowed it down to
I want to research; The Tango Stain, from Anatech and Phoenix Blue
Nuclear Stain, ordered from Fisher.  Is anyone familiar with either of
these stains?  If so, what are the positive/negatives about each one?
Are the results as comparable to the actual Hematoxylin dye?  I have
searched the net for images of a completed stain, to no avail.  Any
information will be greatly appreciated .


Thanks in advance.

Deanna Leslie HT ASCP

Lead Histotechnician

Histology Research Laboratory

Shriners Hospital for Children

Cincinnati, OH 



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