[Histonet] filtering methods

Bruce Abaloz brucea <@t> unimelb.edu.au
Mon Aug 11 23:57:52 CDT 2008

Hi Debra,
We use 'Biopsy pads' which are a blue sponge material (also come in 
green but we find blue shows the tiniest bits best) that will hold 
the tiniest of tissue Bx's (you can also 'colour' your specimen with 
Eosin so it can be seen easier). Be SURE to source a sponge/pad, that 
has a very fine sponge as some we trialled were quite 
'porous'/allowed tiny bits to move into the sponge. Place tissue on 
bottom layer then cover with another pad & close your cassette. Dip 
into 70% ETOH before you drop into formalin/fixative as sponges are 
'hydrophobic' until they have been wetted with ETOH.
Process as usual, remove from paraffin, open cassette & 'voila' - 
there is the Bx.- safe'n'sound!

Histology Facility Manager
The University of Melbourne
Department of Zoology
Victoria. AUSTRALIA 3010
Ph:  (03) 83446282
Fax:(03) 83447909
Email: brucea <@t> unimelb.edu.au
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