[Histonet] Sodium nitrite in fixative during perfusion

[Caroline Bass]

Hello Everyone,

Someone recently suggested that I use a 1% sodium nitrite/0.1M phosphate
buffer solution during perfusion.  I would like to obtain the brain for
immunohistochemistry studies. Are there any opinions on whether the sodium
nitrite is a good reagent to use for these end points?  How does the Na
nitrite work, is it a vasodilator?

Thanks,

Caroline Bass