[Histonet] uneven ihc staining

William Connor will <@t> histologytechservices.com
Sun Aug 10 15:36:25 CDT 2008


If on an autostainer, it could also be the 'probe', which is what tech
support for our autostainer keeps saying...though not likely the case on
ours.

We are in FL and have noticed for the past couple of years inconsistent
staining on DUPLICATE slides has been a problem especially when the temp
rises and the humidity increases around June.  We are currently in the
process of observing this possibility and trying to regulate...

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Friday, August 08, 2008 2:48 PM
To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu; 'richard shook'
Subject: RE: [Histonet] uneven ihc staining

Could also be a sensor issue on an autostainer or if the unit has been moved
recently and not properly levelled.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 08, 2008 9:30 AM
To: histonet <@t> lists.utsouthwestern.edu; richard shook
Subject: Re: [Histonet] uneven ihc staining

Most times this effect is caused by uneven dewaxing of the slides.You should
have a very strict protocol for exchanging the dewaxing reagents.
René J.

--- On Fri, 8/8/08, richard shook <rshooki_99 <@t> yahoo.com> wrote:

From: richard shook <rshooki_99 <@t> yahoo.com>
Subject: [Histonet] uneven ihc staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 8, 2008, 8:50 AM

Im looking for help we are having uneven staining on some (not all) ihc
slides
we have looked into several different possoble reasons with no luck ,this
happens a couple times every week ao so any suggestion would be great 


      

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