[Histonet] uneven ihc staining

[sheila adey]

We had similar issues happening irregularly. We are now discarding the distilled H2O and TRIS buffer that the slides pre soak in before going on the instrument and after dewaxing. We haven't had any complaints since we started this. Sheila Adey HT MLTPort Huron HospitalMichigan> From: b-frederick <@t> northwestern.edu> To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu; rshooki_99 <@t> yahoo.com> Date: Fri, 8 Aug 2008 13:48:24 -0500> Subject: RE: [Histonet] uneven ihc staining> CC: > > Could also be a sensor issue on an autostainer or if the unit has been moved> recently and not properly levelled.> Bernice> > > Bernice Frederick HTL (ASCP)> Northwestern University> Pathology Core Facility> ECOGPCO-RL > 710 N Fairbanks Court> Olson 8-421> Chicago,IL 60611> 312-503-3723> > > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa> Sent: Friday, August 08, 2008 9:30 AM> To: histonet <@t> lists.utsouthwestern.edu; richard shook> Subject: Re: [Histonet] uneven ihc staining> > Most times this effect is caused by uneven dewaxing of the slides.You should> have a very strict protocol for exchanging the dewaxing reagents.> René J.> > --- On Fri, 8/8/08, richard shook <rshooki_99 <@t> yahoo.com> wrote:> > From: richard shook <rshooki_99 <@t> yahoo.com>> Subject: [Histonet] uneven ihc staining> To: histonet <@t> lists.utsouthwestern.edu> Date: Friday, August 8, 2008, 8:50 AM> > Im looking for help we are having uneven staining on some (not all) ihc> slides> we have looked into several different possoble reasons with no luck ,this> happens a couple times every week ao so any suggestion would be great > > > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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