[Histonet] RE: Quick dry coverslipping media
Christine Bark
CBark <@t> memorialcare.org
Wed Aug 6 10:36:17 CDT 2008
We use Mounting Medium (MM) 24 from Surgipath on our automated coverslipper and for hand coverslipping. It works great.
Christine Bark HT(ASCP)
Lead Histotech, Pathology
Saddleback Memorial Medical Center
949-452-3548
cbark <@t> memorialcare.org
-----Original Message-----
We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
------------------------------
Message: 5
Date: 05 Aug 2008 13:38:37 -0400
From: Elizabeth.A.Allen <@t> Hitchcock.ORG (Elizabeth A. Allen)
Subject: [Histonet] question regarding crystal violet stain......
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42188051 <@t> mailbox4.Hitchcock.ORG>
Content-Type: text/plain; charset=iso-8859-1
I am trying to find a procedure for staining fresh muscle tissue for crystal violet to demonstrate amyloid.
Does anyone have a protocol they could send me?
Thank you.
Beth
------------------------------
Message: 6
Date: Tue, 5 Aug 2008 13:41:56 -0400
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Quick dry coverslipping media
To: "Tom McNemar" <TMcNemar <@t> lmhealth.org>,
<histonet <@t> pathology.swmed.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF8900BAF854C <@t> smcmail.somerset-healthcare.com>
Content-Type: text/plain; charset="utf-8"
We use Richard-Allan mounting medium and file slides the next day. Catalog #4111
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
McNemar
Sent: Tuesday, August 05, 2008 1:33 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Quick dry coverslipping media
We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 7
Date: Tue, 05 Aug 2008 17:48:05 +0000
From: zodiac29 <@t> comcast.net
Subject: [Histonet] Alcian blue/PAS
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<080520081748.21750.48989255000CB90F000054F62216554886C7CD0C0E070B0196 <@t> comcast.net>
Content-Type: text/plain
Hello All,
I wanted to know what variation of the alcian blue/PAS stain that you like best. We are going to start doing this stain instead of the alcian blue that we do now. The texts that I use (Carson, Bancroft, AFIP) all have diffrent variations. I just wanted some imput on what is best.
Thank you
Jenny
------------------------------
Message: 8
Date: Tue, 5 Aug 2008 13:57:58 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] RE: Quick dry coverslipping media
To: "'Rathborne, Toni'" <trathborne <@t> somerset-healthcare.com>, Tom
McNemar <TMcNemar <@t> lmhealth.org>, "histonet <@t> pathology.swmed.edu"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E390B77E35D <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
I use that as well and have no problem with next day filing.
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek <@t> digestivespecialists.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Tuesday, August 05, 2008 1:42 PM
To: Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
We use Richard-Allan mounting medium and file slides the next day. Catalog #4111
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
McNemar
Sent: Tuesday, August 05, 2008 1:33 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Quick dry coverslipping media
We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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printing, copying, distribution, or use of such information is strictly
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promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
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Somerset Medical Center is proud to receive the Somerset County
Business Partnership's 2006 Quality of Life Award.
------------------------------
Message: 9
Date: Tue, 5 Aug 2008 13:52:25 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Quick dry coverslipping media
To: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>, "Tom
McNemar" <TMcNemar <@t> lmhealth.org>, <histonet <@t> pathology.swmed.edu>
Message-ID:
<982A0A9461F9BF438C7B19A6E425A3834AFB0D <@t> ITSSSXM01V6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
Does it work well with automatic coverslippers?
Thanks, j
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Tuesday, August 05, 2008 1:42 PM
To: Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
We use Richard-Allan mounting medium and file slides the next day.
Catalog #4111
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
McNemar
Sent: Tuesday, August 05, 2008 1:33 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Quick dry coverslipping media
We are being buried in slides. We are currently using Cytoseal 60 from
Richard Alan as a coverslipping media but it takes a long time for it to
dry. Does anyone knkow of a coverslipping media that would allow us to
file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Center and are intended only for the addressee. The information
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Unauthorized forwarding, printing, copying, distribution, or use of such
information is strictly prohibited and may be unlawful. If you are not
the addressee, please promptly delete this message and notify the sender
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Center's computer Help Desk at 908-685-2200, ext. 4050.
Somerset Medical Center is proud to receive the Somerset County Business
Partnership's 2006 Quality of Life Award.
Confidentiality Notice:
This email, including any attachments is the
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It may contain information that is privileged and
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sender that you have received the message in
error, then delete this message.
------------------------------
Message: 10
Date: Tue, 5 Aug 2008 14:10:17 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: RE: [Histonet] Quick dry coverslipping media
To: "'Weems, Joyce'" <JWeems <@t> sjha.org>, "Rathborne, Toni"
<trathborne <@t> somerset-healthcare.com>, Tom McNemar
<TMcNemar <@t> lmhealth.org>, "histonet <@t> pathology.swmed.edu"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E390B77E35F <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
I use it with my coverslipper.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, August 05, 2008 1:52 PM
To: Rathborne, Toni; Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
Does it work well with automatic coverslippers?
Thanks, j
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Tuesday, August 05, 2008 1:42 PM
To: Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
We use Richard-Allan mounting medium and file slides the next day.
Catalog #4111
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
McNemar
Sent: Tuesday, August 05, 2008 1:33 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Quick dry coverslipping media
We are being buried in slides. We are currently using Cytoseal 60 from
Richard Alan as a coverslipping media but it takes a long time for it to
dry. Does anyone knkow of a coverslipping media that would allow us to
file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical
Center and are intended only for the addressee. The information
contained in this message is confidential and may contain privileged,
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protection and/or exemption from disclosure under applicable law.
Unauthorized forwarding, printing, copying, distribution, or use of such
information is strictly prohibited and may be unlawful. If you are not
the addressee, please promptly delete this message and notify the sender
of the delivery error by e-mail or you may call Somerset Medical
Center's computer Help Desk at 908-685-2200, ext. 4050.
Somerset Medical Center is proud to receive the Somerset County Business
Partnership's 2006 Quality of Life Award.
Confidentiality Notice:
This email, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
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sender that you have received the message in
error, then delete this message.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 5 Aug 2008 14:03:09 -0400
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Quick dry coverslipping media
To: "Weems, Joyce" <JWeems <@t> sjha.org>, "Tom McNemar"
<TMcNemar <@t> lmhealth.org>, <histonet <@t> pathology.swmed.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF8900BAF854E <@t> smcmail.somerset-healthcare.com>
Content-Type: text/plain; charset="utf-8"
Yes. We use it on our Leica CV5030.
-----Original Message-----
From: Weems, Joyce [mailto:JWeems <@t> sjha.org]
Sent: Tuesday, August 05, 2008 1:52 PM
To: Rathborne, Toni; Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
Does it work well with automatic coverslippers?
Thanks, j
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Tuesday, August 05, 2008 1:42 PM
To: Tom McNemar; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Quick dry coverslipping media
We use Richard-Allan mounting medium and file slides the next day.
Catalog #4111
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tom
McNemar
Sent: Tuesday, August 05, 2008 1:33 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Quick dry coverslipping media
We are being buried in slides. We are currently using Cytoseal 60 from
Richard Alan as a coverslipping media but it takes a long time for it to
dry. Does anyone knkow of a coverslipping media that would allow us to
file our slides within a couple of days without them sticking together?
Thanks!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical
Center and are intended only for the addressee. The information
contained in this message is confidential and may contain privileged,
confidential, proprietary and/or trade secret information entitled to
protection and/or exemption from disclosure under applicable law.
Unauthorized forwarding, printing, copying, distribution, or use of such
information is strictly prohibited and may be unlawful. If you are not
the addressee, please promptly delete this message and notify the sender
of the delivery error by e-mail or you may call Somerset Medical
Center's computer Help Desk at 908-685-2200, ext. 4050.
Somerset Medical Center is proud to receive the Somerset County Business
Partnership's 2006 Quality of Life Award.
Confidentiality Notice:
This email, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please reply to the
sender that you have received the message in
error, then delete this message.
CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee. The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law. Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful. If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.
Somerset Medical Center is proud to receive the Somerset County
Business Partnership's 2006 Quality of Life Award.
------------------------------
Message: 12
Date: Tue, 5 Aug 2008 14:50:03 -0400
From: "Pumla Pamla-Gutter" <pumla.pamla <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<cd2e36510808051150g55056699qd2ab78e307b4a395 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear All,
Any feedback appreciated.
I've always used Cresyl Violet Echt, and now I've just switched to
Cresyl Violet acetate. Does anyone know if I could treat these two the
same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does
anyone work with this Nissl body stain and if so what protocol do you
use?
Thank you.
Pumla
------------------------------
Message: 13
Date: Tue, 5 Aug 2008 13:21:53 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Cost per test
To: histonet <@t> lists.utsouthwestern.edu, Kari Breal <BrealK <@t> alexian.net>
Message-ID: <286694.63074.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Under separate cover I am sending you an article I wrote on the subject.
René J.
--- On Tue, 8/5/08, Kari Breal <BrealK <@t> alexian.net> wrote:
From: Kari Breal <BrealK <@t> alexian.net>
Subject: [Histonet] Cost per test
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, August 5, 2008, 1:15 PM
Does anyone have a cost per slide/block formula for histology work they would be
willing to share?
Thanks,
Kari Breal
Histology Supervisor
Alexian Brothers Medical Center
847-437-5500 ext. 5155
Fax 847-981-2023
brealk <@t> alexian.net
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential and
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is prohibited. If you are not the intended recipient, please contact the sender
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------------------------------
Message: 14
Date: Tue, 5 Aug 2008 17:41:43 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: [Histonet] computer system volunteers?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00bb01c8f744$1f138970$5d3a9c50$@com>
Content-Type: text/plain; charset="us-ascii"
Good evening,
I'm looking for some volunteers to give us some input on billing features
that you absolutely love or would love to have in your system. Basically,
we're enhancing the billing aspect of our AP system and we'd like to get as
many opinions as possible. Of course, we've already started with our
current clients, but we'd like to expand our horizons.
So, if you don't mind sharing some of the best parts, worst parts, or even
fantasies, I'd appreciate it.
To start with, our overall objective is to make billing as automatic as
possible. Why we involve our technologists or pathologists in billing has
always been beyond me. Your jobs should be to focus on performing the task
as hand, and to me, at least, that's not about billing.
...though I know we have to bill to stay in existence.
I appreciate whatever you can give me.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pumla
Pamla-Gutter
Sent: Tuesday, August 05, 2008 2:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9
Dear All,
Any feedback appreciated.
I've always used Cresyl Violet Echt, and now I've just switched to
Cresyl Violet acetate. Does anyone know if I could treat these two the
same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does
anyone work with this Nissl body stain and if so what protocol do you
use?
Thank you.
Pumla
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Tue, 5 Aug 2008 20:07:50 EDT
From: BERGERJL <@t> aol.com
Subject: [Histonet] Horse bones
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c8e.277d1ae9.35ca4556 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
We are trying to section pieces of horse femurs, fetlock and carpal joints.
They have been decalcified with either HCl decal or formic acid decal.
Before routine processing the bones appear to be decalcified, but at sectioning
they are very hard and brittle (even after surface decal) and chip out of the
paraffin block.
Any suggestions would be helpful. A pathologist suggested a soap soaking
solution but could not remember the name of the soap.
Help and thank you,
R. Berger, HT
**************Looking for a car that's sporty, fun and fits in your budget?
Read reviews on AOL Autos.
(http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 )
------------------------------
Message: 16
Date: Wed, 6 Aug 2008 12:14:36 +1000
From: "Young Kwun" <kwuny <@t> email.cs.nsw.gov.au>
Subject: [Histonet] Factor XIIIa antibody
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <200808061214847.SM00940 <@t> csls2816>
Content-Type: text/plain; charset="us-ascii"
Hello All,
We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem
(Cat.No.233498) on skin conditions successfully so far.
However this antibody was discontinued few years ago and they are offering
Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit
immunostaining on human skin, I tried two other monoclonal Factor XIIIa but
the stains were not as good as our current one.
I tried Neomaker and Novocastra's. Can anyone suggest the source of good
FXIIIa please? Thank you.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
------------------------------
Message: 17
Date: Wed, 6 Aug 2008 14:05:56 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "Bill" <bill501 <@t> mindspring.com>, <Pathrm35 <@t> comcast.net>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FAFFBC <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
If you like, I can come and clean the lenses, at a modest price (say
10c/hr) plus travelling.
My microscope lenses always look worse after I clean them, so I should
have the same success with your camera lenses.
By the way, I live in Sydney, Australia!!
But seriously, what a dumb move, to install cameras without
consultation!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bill
Sent: Tuesday, 5 August 2008 12:02 AM
To: Pathrm35 <@t> comcast.net; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] surveillance cameras in the lab
At 10:21 AM +0000 8/4/08, Pathrm35 <@t> comcast.net wrote:
>>>I was wondering how many techs out there have cameras in their labs,
>>>either for security or to monitor employees. I went to work Sunday
>>>night and noticed that 4 cameras were installed in the lab over the
>>>weekend, with more to come.<<<
I would use immersion oil on the camera lenses.
--
______________
Bill Blank, MD
Heartland Lab
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Message: 18
Date: Wed, 6 Aug 2008 14:31:10 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Factor XIIIa antibody
To: "Young Kwun" <kwuny <@t> email.cs.nsw.gov.au>, "Histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FAFFBD <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Hi Young,
How is my old lab going?
Try Biogenix Cat No: MU337-UP
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Young
Kwun
Sent: Wednesday, 6 August 2008 12:15 PM
To: Histonet
Subject: [Histonet] Factor XIIIa antibody
Hello All,
We've been using Factor XIII, A-subunit polyclonal antibody from
Calbiochem
(Cat.No.233498) on skin conditions successfully so far.
However this antibody was discontinued few years ago and they are
offering Factor XIII, S-subunit only. Although I haven't tried FXIII
S-unit immunostaining on human skin, I tried two other monoclonal Factor
XIIIa but the stains were not as good as our current one.
I tried Neomaker and Novocastra's. Can anyone suggest the source of good
FXIIIa please? Thank you.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************
------------------------------
Message: 19
Date: Tue, 05 Aug 2008 23:43:32 -0500 (CDT)
From: Steven Hacker <stevenhacker <@t> verizon.net>
Subject: Re: RE: [Histonet] Factor XIIIa antibody
To: Tony Henwood <AnthonyH <@t> chw.edu.au>, Histonet
<histonet <@t> pathology.swmed.edu>, Young Kwun <kwuny <@t> email.cs.nsw.gov.au>
Message-ID:
<18418115.7124031217997812488.JavaMail.root <@t> vms068.mailsrvcs.net>
Content-Type: text/plain; charset=UTF-8
Please take a look at BioCare Medical's Factor XIIIa Ab...
=====================
From: Tony Henwood <AnthonyH <@t> chw.edu.au>
Date: 2008/08/06 Wed AM 12:31:10 EDT
To: Young Kwun <kwuny <@t> email.cs.nsw.gov.au>,
Histonet <histonet <@t> pathology.swmed.edu>
Subject: RE: [Histonet] Factor XIIIa antibody
Hi Young,
How is my old lab going?
Try Biogenix Cat No: MU337-UP
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Young
Kwun
Sent: Wednesday, 6 August 2008 12:15 PM
To: Histonet
Subject: [Histonet] Factor XIIIa antibody
Hello All,
We've been using Factor XIII, A-subunit polyclonal antibody from
Calbiochem
(Cat.No.233498) on skin conditions successfully so far.
However this antibody was discontinued few years ago and they are
offering Factor XIII, S-subunit only. Although I haven't tried FXIII
S-unit immunostaining on human skin, I tried two other monoclonal Factor
XIIIa but the stains were not as good as our current one.
I tried Neomaker and Novocastra's. Can anyone suggest the source of good
FXIIIa please? Thank you.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Wed, 6 Aug 2008 09:58:41 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] Horse bones
To: "BERGERJL <@t> aol.com" <BERGERJL <@t> aol.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0808060058x60c690d9y30d015efa8facb25 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Some things to think about....
Rinse the tissue well with running tap water after decal and before
processing - this seems to make a difference (depends on the size of the
bone - 2hrs to overnight)
EMbed the bones so that the long axis is either at right angles or 45
degrees to the blade so that the edge of the knife doesn't have to cut
through a narrow band of cortical bone along its entire length - this causes
"skipping"(If this is unclear I can send you an attachment with a diagramme)
Once you have faced the blocks put the face down on ice in a -20 deg freezer
overnight (chest type freezer). then remove the one ata time for sectioning.
I have only had limited sucess with soaking the faced blocks in fabric
softener undiluted.
Hopes this helps
best regards
On 8/6/08, BERGERJL <@t> aol.com <BERGERJL <@t> aol.com> wrote:
>
> We are trying to section pieces of horse femurs, fetlock and
> carpal joints.
> They have been decalcified with either HCl decal or formic acid decal.
> Before routine processing the bones appear to be decalcified, but at
> sectioning
> they are very hard and brittle (even after surface decal) and chip out of
> the
> paraffin block.
> Any suggestions would be helpful. A pathologist suggested a soap soaking
> solution but could not remember the name of the soap.
>
> Help and thank you,
>
> R. Berger, HT
>
>
>
>
> **************Looking for a car that's sporty, fun and fits in your budget?
> Read reviews on AOL Autos.
> (
> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 21
Date: Wed, 6 Aug 2008 18:42:48 +1000
From: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
Subject: Re: [Histonet] Horse bones
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006801c8f7a0$67fd9840$d175be7c <@t> pentium4>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
For tough or brittle, but not necessarily calcified tissue, I find that
soaking the block on a water soaked tissue for 10-15 minutes effective for
softening. It can sometimes take all day to get a section, as trimming
sometimes has to be done 4-5 microns at a time!!
Piero Nelva
Monash Medical Centre
Victoria
Australia
----- Original Message -----
From: "louise renton" <louise.renton <@t> gmail.com>
To: <BERGERJL <@t> aol.com>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 06, 2008 5:58 PM
Subject: Re: [Histonet] Horse bones
> Some things to think about....
>
> Rinse the tissue well with running tap water after decal and before
> processing - this seems to make a difference (depends on the size of the
> bone - 2hrs to overnight)
>
> EMbed the bones so that the long axis is either at right angles or 45
> degrees to the blade so that the edge of the knife doesn't have to cut
> through a narrow band of cortical bone along its entire length - this
> causes
> "skipping"(If this is unclear I can send you an attachment with a
> diagramme)
>
> Once you have faced the blocks put the face down on ice in a -20 deg
> freezer
> overnight (chest type freezer). then remove the one ata time for
> sectioning.
>
> I have only had limited sucess with soaking the faced blocks in fabric
> softener undiluted.
>
> Hopes this helps
>
> best regards
>
>
> On 8/6/08, BERGERJL <@t> aol.com <BERGERJL <@t> aol.com> wrote:
>>
>> We are trying to section pieces of horse femurs, fetlock and
>> carpal joints.
>> They have been decalcified with either HCl decal or formic acid decal.
>> Before routine processing the bones appear to be decalcified, but at
>> sectioning
>> they are very hard and brittle (even after surface decal) and chip out
>> of
>> the
>> paraffin block.
>> Any suggestions would be helpful. A pathologist suggested a soap
>> soaking
>> solution but could not remember the name of the soap.
>>
>> Help and thank you,
>>
>> R. Berger, HT
>>
>>
>>
>>
>> **************Looking for a car that's sporty, fun and fits in your
>> budget?
>> Read reviews on AOL Autos.
>> (
>> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017)
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> No virus found in this incoming message.
> Checked by AVG - http://www.avg.com
> Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 8/5/2008
> 9:49 PM
>
>
>
------------------------------
Message: 22
Date: Wed, 6 Aug 2008 07:33:31 -0500
From: "Drew Sally A." <SDrew <@t> uwhealth.org>
Subject: RE: [Histonet] Factor XIIIa antibody
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<3F328377AF4E23438E78234752652CE105D52772 <@t> uwhis-xchng7.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="US-ASCII"
I'f you are at all interested in a polyclonal antibody, we have been
happy with BioCare Medical's F13a antibody, and I would be happy to tell
you more about it if you desire.
Sally Ann Drew, MT (ASCP)
sdrew <@t> uwhealth.org
IHC/ISH Lab DB1-223, Mail Code 3224
600 Highland Ave.
Madison, WI 53792
Phone (608) 265-6596
Fax (608) 262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Young
Kwun
Sent: Tuesday, August 05, 2008 9:15 PM
To: Histonet
Subject: [Histonet] Factor XIIIa antibody
Hello All,
We've been using Factor XIII, A-subunit polyclonal antibody from
Calbiochem
(Cat.No.233498) on skin conditions successfully so far.
However this antibody was discontinued few years ago and they are
offering
Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit
immunostaining on human skin, I tried two other monoclonal Factor XIIIa
but
the stains were not as good as our current one.
I tried Neomaker and Novocastra's. Can anyone suggest the source of good
FXIIIa please? Thank you.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 57, Issue 10
****************************************
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