[Histonet] Re: Rabbit IgG control
R C
ruebenjcarter <@t> gmail.com
Mon Aug 4 11:25:07 CDT 2008
Jackson Immuno Research.
On Sat, Aug 2, 2008 at 10:01 AM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
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> Today's Topics:
>
> 1. Rabbit IgG control (Margaryan, Naira)
> 2. Alginate beads containing cells (Danielson, Keith)
> 3. HT schools (Amber McKenzie)
> 4. Christopher Hayden is nowhere near the lab.
> (christopher.hayden <@t> novartis.com)
> 5. A question for BondMax users (Victoria Baker)
> 6. Re: HT schools (Victoria Baker)
> 7. RE: A question for BondMax users (Beckham, Sharon)
> 8. Bone Marrow Biopsy Needles (Dana Brewer)
> 9. Re: HT schools (Rene J Buesa)
> 10. processing PFA fixed alginate beads (Gayle Callis)
> 11. Methodology for extracting nucleic acids from FFPE Tissue
> (Gayle Callis)
> 12. RE: Dictation systems (Michael Mihalik)
> 13. Re: HT schools (Joe Nocito)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 1 Aug 2008 12:03:09 -0500
> From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
> Subject: [Histonet] Rabbit IgG control
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> EE5AF506236CEE4CAFD1A281F5DCFE90021035C8 <@t> CMHEXC02EVS.childrensmemorial.org
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Dears,
>
>
>
> I have to order a Rabbit IgG control. What company is making best Rabbit
> IgG control, not universal, please.
>
>
>
> Thanks in advance,
>
> Naira
>
>
>
> Naira V. Margaryan, D.V.M., Ph.D.
>
> Research Scientist
>
> Children's Memorial Research Center
>
> 2300 Children's Plaza, Box 222
>
> Chicago, IL 60614-3363
>
> Tel: 773-755-6340
>
> Fax: 773-755-6594
>
> nmargaryan <@t> childrensmemorial.org
> <mailto:nmargaryan <@t> childrensmemorial.org>
>
>
>
> For Express Mail:
>
> CMRC, Room C.473
>
> 2430 N. Halsted Street
>
> Chicago, IL 60614-4314
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 1 Aug 2008 13:07:21 -0400
> From: "Danielson, Keith" <Keith.Danielson <@t> uphs.upenn.edu>
> Subject: [Histonet] Alginate beads containing cells
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <84D11048BB18234D8A56474645E8EC020829EF6F <@t> uphsmbx5.UPHS.PENNHEALTH.PRV>
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello everyone,
>
>
>
> I am requesting information for a graduate student on how to fix and
> process alginate beads with embedded tissue culture cells for paraffin
> histology. The beads are fixed in 4% paraformaldehyde but seem to turn
> white and disintegrate during routine processing.
>
>
>
> Thanks very much,
>
>
>
> Keith Danielson, PhD
>
> Supervisor, IHC Lab
>
> Pennsylvania Hospital
>
> Philadelphia, PA 19107
>
>
>
> Phone: 215 829-7726 or 829-3696
>
> E-mail: keith.danielson <@t> uphs.upenn.edu
>
>
>
>
>
>
>
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>
> ------------------------------
>
> Message: 3
> Date: Fri, 1 Aug 2008 12:23:42 -0500
> From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF <@t> giamail2.Gia.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Where are all the HT accredited schools and why aren't there more out
> there? I've seen the online classes' people can take, but that requires
> them to be trained in a lab, as well, for the "hands on" part. So,
> actually the supervisor still has to train potential HT's "on the job"
> before they can sit for the board exam. Right?
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 1 Aug 2008 13:41:00 -0400
> From: christopher.hayden <@t> novartis.com
> Subject: [Histonet] Christopher Hayden is nowhere near the lab.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> OFC6859B0A.C0BE57E7-ON85257498.0061233C-85257498.0061233C <@t> ah.novartis.com>
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting 08/01/2008 and will not return until
> 08/11/2008.
>
> Good Day Everyone!
>
> I will be out of the lab from 2 August until 10 August. I'll be back at my
> desk on the morning of the 11th. I will have no access to corporate email
> during this time.
>
> If you have an EM-related matter, please contact the Lab Manager, Gregory
> Argentieri, at x2-8617
>
> Otherwise, I'll get back to you on the 11th or shortly afterwards.
>
> Thanks!
> -CH
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 1 Aug 2008 14:13:13 -0400
> From: "Victoria Baker" <bakevictoria <@t> gmail.com>
> Subject: [Histonet] A question for BondMax users
> To: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4f016b690808011113k4bceed66w3a76c8bb38fa8cb <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Happy Friday everyone!
>
> In the BondMax detection system there is a "post primary" step that
> contains 10% BSA in a Tris buffer. I've tested the protocol excluding
> this step and did not have any staining.
>
> I can't seem to get the answer I'm looking for from the company, so if
> anyone can help me I'd really appreciate it.
>
> Thanks
>
> Vikki
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 1 Aug 2008 14:18:43 -0400
> From: "Victoria Baker" <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] HT schools
> To: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <4f016b690808011118k34ecf6c5r20c4960d00b679c6 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Amber,
>
> If you go to the NSH website there are schools listed there. I'm an
> alumni of the SUNY school at Cobleskill - and I loved it - but it was
> also thirty years ago.
>
> I would check that list and see if you can't make some calls or
> e-mails to their co-ordinators.
>
> Regards,
>
> Vikki Baker
>
>
>
> On 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
> > Where are all the HT accredited schools and why aren't there more out
> > there? I've seen the online classes' people can take, but that requires
> > them to be trained in a lab, as well, for the "hands on" part. So,
> > actually the supervisor still has to train potential HT's "on the job"
> > before they can sit for the board exam. Right?
> >
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 1 Aug 2008 13:27:36 -0500
> From: "Beckham, Sharon" <SLB <@t> Stowers-Institute.org>
> Subject: RE: [Histonet] A question for BondMax users
> To: 'Victoria Baker' <bakevictoria <@t> gmail.com>, Histo Net list server
> <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> BD62CBAC4395B94096109020651BE2EC129EDDCE2F <@t> exchmb-02.stowers-institute.org
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Vikki,
>
> I don't know if I'm much help on this because I just started testing the
> BondMax this week so I'm just in the learning process too. As far as I can
> tell the post primary is for blocking, but also works in conjunction with
> the polymer which is the following step. It supposedly also enhances
> penetration of the polymer, so they both have to be used together. Hope
> this helps.
>
> Sharon
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria Baker
> Sent: Friday, August 01, 2008 1:13 PM
> To: Histo Net list server
> Subject: [Histonet] A question for BondMax users
>
>
> Happy Friday everyone!
>
> In the BondMax detection system there is a "post primary" step that
> contains 10% BSA in a Tris buffer. I've tested the protocol excluding this
> step and did not have any staining.
>
> I can't seem to get the answer I'm looking for from the company, so if
> anyone can help me I'd really appreciate it.
>
> Thanks
>
> Vikki
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 1 Aug 2008 13:27:28 -0500
> From: "Dana Brewer" <brewerd <@t> nacmem.org>
> Subject: [Histonet] Bone Marrow Biopsy Needles
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000601c8f404$619b66c0$0b0a10ac <@t> work.nmh.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone
> Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the
> replacement we got is not the same "marrow loc" system our oncologist
> prefers.
> Thanks for your help
>
> ------------------------------
>
> Message: 9
> Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu, Amber McKenzie
> <amber.mckenzie <@t> gastrodocs.net>
> Message-ID: <78031.12752.qm <@t> web65716.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Wrong! The advantage of the "on line" or "distance learning" courses is
> that they provide the theory on line while you are working at a given
> laboratory doing your training (or even as part of your daily work) so there
> is no "actual training" to be done by the supervisor.
> At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an
> overall capacity of about 300-325 students, and this will not be enough to
> take care of all the retiring histotechs.
> Costs is one of the reasons why the number of HTs schools is dwindling.
> René J.
>
>
> --- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
>
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, August 1, 2008, 1:23 PM
>
> Where are all the HT accredited schools and why aren't there more out
> there? I've seen the online classes' people can take, but that
> requires
> them to be trained in a lab, as well, for the "hands on" part. So,
> actually the supervisor still has to train potential HT's "on the
> job"
> before they can sit for the board exam. Right?
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 1 Aug 2008 15:23:31 -0600
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] processing PFA fixed alginate beads
> To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000801c8f41c$d9196180$6401a8c0 <@t> Sunney>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I Googled this subject and brought up several publications using alginate
> beads embedded in paraffin. HOwever, the following publication, using
> cryotomy, had better results than using paraffin sections.
>
> Also, Yang C-C, et al. Adapted cyrosectioning method for hydrogels used in
> regenerative medicine. J Histotechnology 30(3):185-191, 2007. Linda
> Jenkins, on of the co-authors, was a huge part of this, has much experience
> with hydrogels. In this publication, they fabricated their own alginate
> beads. The reference list is extensive and informative.
>
> Good Luck
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
>
>
>
> --------------------------------------------------------------------------------
>
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 1 Aug 2008 15:28:53 -0600
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] Methodology for extracting nucleic acids from FFPE
> Tissue
> To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000f01c8f41d$990a30f0$6401a8c0 <@t> Sunney>
> Content-Type: text/plain; charset="iso-8859-1"
>
> For the person needing a protocol for extracting DNA and/or RNA from breast
> (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from
> Epicenter Biotechnologies, www. EpiBio.com
>
> Phone # is 800 284-8474.
>
> This information was found in the latest issue of Science ( Life ScienCe
> Technologies section)
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 1 Aug 2008 18:14:56 -0400
> From: "Michael Mihalik" <mike <@t> pathview.com>
> Subject: RE: [Histonet] Dictation systems
> To: <ht.ascp <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001501c8f424$204a2830$60de7890$@com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Jon, did you ever get an answer to this question?
>
> I am not as familiar with all the various dictation systems that I would
> like to be, but I have used something that may help.
>
> You can buy a foot pedal that can be 'programmed' to send a hotkey
> combination to an external device, typically a PC.
>
> In my case, I was considering using this setup to enable and disable
> Dragon
> Naturally Speaking at the gross workstation.
>
> Michael Mihalik
> PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jon Google
> Sent: Tuesday, July 29, 2008 3:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Dictation systems
>
> We are in the process of upgrading our LIS. We are looking at moving our
> dictation system from our current Dictaphone analog system. We would like
> to
> use a digital system that can support VOIP. The problem we are running
> into
> is trying to find one that supports a hands free control that our grossing
> pathologists need.
>
> Does anyone have recommendations on digital systems that support foot
> controls?
>
> Thanks
> Jon
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Sat, 2 Aug 2008 07:19:36 -0500
> From: "Joe Nocito" <jnocito <@t> satx.rr.com>
> Subject: Re: [Histonet] HT schools
> To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>, "Amber
> McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Message-ID: <002c01c8f49a$07995790$e797b348 <@t> yourxhtr8hvc4p>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> our one community college program in San Antonio is in jeopardy of closing
> because it can't get the required 10 students to make a class. If this
> program does close, I will be willing to work with a college or university
> with long- distance learning. I have 5 openings in my lab.
>
> JTT
> ----- Original Message -----
> From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
> To: <histonet <@t> lists.utsouthwestern.edu>; "Amber McKenzie"
> <amber.mckenzie <@t> gastrodocs.net>
> Sent: Friday, August 01, 2008 2:42 PM
> Subject: Re: [Histonet] HT schools
>
>
> Wrong! The advantage of the "on line" or "distance learning" courses is
> that
> they provide the theory on line while you are working at a given laboratory
> doing your training (or even as part of your daily work) so there is no
> "actual training" to be done by the supervisor.
> At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an
> overall capacity of about 300-325 students, and this will not be enough to
> take care of all the retiring histotechs.
> Costs is one of the reasons why the number of HTs schools is dwindling.
> René J.
>
>
> --- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
>
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, August 1, 2008, 1:23 PM
>
> Where are all the HT accredited schools and why aren't there more out
> there? I've seen the online classes' people can take, but that
> requires
> them to be trained in a lab, as well, for the "hands on" part. So,
> actually the supervisor still has to train potential HT's "on the
> job"
> before they can sit for the board exam. Right?
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 57, Issue 2
> ***************************************
>
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