[Histonet] paraffin mouse femur peroist detaching from slide

[mesruh turkekul]

Dear Friends,

I have fixed and decalcified  mouse femur with EDTA and paraffin embedded.
The sectioning was perfect and also the H&E looked very good. I used super
frost/plus slides and baked the sections at 56C  1 hour.
After IHC on Ventana machines the periost was gone but the bone marrow was
perfectly stable on the sections.
Any suggestions?


Mesru Turkekul

MSKCC.org
New York, NY