[Histonet] need help with PAP stain

[Liz Chlipala]

Hello everyone
 
I need to run a pap stain on some saliva samples and I don't know where
to begin.  I know this seems like a silly question, but I have done some
searching on the net and there are quite a few protocols with different
reagents.  Its not a large project for us, I only need to run about 30
to 40 slides and was hoping to purchase a kit that would contain the
hematoxylin, OG-6 and EA-50 but the only one I can find is the one from
thermo the rapid chrome pap staining kit but I can't seem to find a
vendor that sells it.  If possible I would like to purchase pints of
these reagents rather than gallons. Any advice as to what reagents to
purchase and a protocol would be helpful.  Also advice on what the stain
should look like would help too.
 
Thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/> 
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504