[Histonet] IHC Staining Variations

[Weber, Susan (VHACLE)]

Trinity-
Are you using the Ventana Benchmark XT equipment? I recently started
using this equipment and in our training class they told us that certain
slides can be hydrophobic and using a Pap Pen can also effect the
staining quality. I switched from Surgipath X slides to Fisher
Superfrost/Plus slides for this very reason. I use the Shandon Double
slide with the rings on them for our FITC stains and did not have a
problem - the ring is on the underside of the slide, not the side you
put the tissue on. Some of the slides which have "boxes" on them also
affect staining quality (unless it is on the underside of the slide of
course). If you are using the Ventana equipment, their Tech Support is
very good and can help you. Even if your not using this equipment, I'd
call the Tech support of your manufacturer and ask for their help.


Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154

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Sent: Thursday, April 17, 2008 2:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Staining Variations

Hello,

We have an automated IHC stainer and the doctors are experiencing what
they call, staining variations.? The whole tissue is not staining, just
in parts, when areas of the tissues should be staining.? 

Has anyone experienced this problem and if so, what did you do to remedy
it?? We were staining manually and used a Pap Pen before we switched to
automation and I'm wondering if we are getting areas on the slide that
are not being covered with the reagents.? We have 3 drop zones with
100ul of reagent dropping in each zone, for a total of 300ul per slide.

I appreciate any feedback and help.? 

Trinity Whitecomb
Histology Manager
Lab System Works
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