[Histonet] Snap Freezing of Lymph Nodes for RNA testing

[Norm Burnham]

Hi Gayle,
Thank you for your advice. We want to avoid LN and Isopentane if at all
possible.  We are looking for a simple, inexpensive, easy to use, and RNAse
free method to preserve, stabilize and transport tissue for RNA studies.
Does anyone have an alternative to LN or Isopentane bath for snap freezing
followed by shipment on dry ice, or an alternate way, to stabilize tissue for
periods ranging from a few hours to perhaps as long as 72 hours?  
Thanks and best regards,
Norm Burnham

----- Original Message -----
From: Gayle Callis <gayle.callis <@t> bresnan.net>
To: Norm Burnham; histonet <@t> lists.utsouthwestern.edu
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wed Apr 16 19:20:36 2008
Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing

It may be better to use a transportable Dewar flask for just the liquid 
nitrogen.  I don't think a doctors office will want to handle isopentane, 
too volatile, flammable and explosive.  Contact or maybe those on Histonet 
in dermatopathology offices have ways to do this for biopsies they collect, 
freeze and ship.

Good luck

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Norm Burnham" <Norm.Burnham <@t> propathlab.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 16, 2008 12:50 PM
Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing


Hi Histonet,



Does anyone have a simple, inexpensive and very portable method for snap
freezing lymph nodes in preparation for shipment on dry ice?  We have
considered Liquid Nitrogen and Isopentane bath, but I am looking for a fully
portable method that can be handled in a doctors office by their staff.



Thanks,



Norm Burnham



____________________________________

Norm Burnham
Director of Laboratory Operations
ProPath - The Leader in Pathology Services(r)

8267 Elmbrook Drive, Suite 100
Dallas, TX  75247
214.237.1602 Office
214.237.1802 Fax

214.709.7127 Cell

Email:  norm.burnham <@t> propath.com <mailto:norm.burnham <@t> propath.com>


To learn more about ProPath, please visit www.ProPath.com
<http://www.ProPath.com>

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