[Histonet] background of H&E staining

[Piero Nelva]

We had a batch of mounting medium that went yellow, and consequently the 
section went yellow too (so long ago that I've forgotten the brand).  Since 
switching to Eukitt, the preparation is much more stable.

Piero Nelva
Histology
Monash Medical Centre
Australia
----- Original Message ----- 
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 16, 2008 6:21 AM
Subject: [Histonet] background of H&E staining


> Hi, all
>
>  Our PI complained about our H&E staining recently. He found the slides we 
> did for him changed color(within a month), the whole slides turn to 
> yellow, although the staining is still there and looks good. I searched 
> for some websites for the answer, but could not find it.
>
>  Does anyone know the reason why the background of slides changed color? 
> We did it in the general way which worked well in the past. And the 
> reagents are not expired. Any suggestions?
>
>  Thanks,
>
> Ann
>
>
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