[Histonet] recommendation for skin and biopsy-processing

[Rene J Buesa]

With such a period in NBF before starting the protocol, you can skip the second NBF station, and add at the end another white spirit station to clear the specimens better.
  René J.

Gudrun Lang <gu.lang <@t> gmx.at> wrote:
  Hi,

I want to implement a new processing protocol on the VIP 5 for biopsies and
skin. Please tell me your comments, if the protocol would be sufficient for
eg. 100 cassettes with biopsy-sponges and fifty cassettes with
skin-specimen. 

The program starts at 11 p.m., the specimen sit in NBF from afternoon till
the start.

30 min NBF - 30 min NBF - 30 min 70% - 30 min 80% - 30 min 96% - 40 min 96%
- 40 min 100% - 40 min 100% - 30 min shell sol (clearing medium) - 40 min
shell sol - 30 min Paraffin - 30 min paraffin - 40 min paraffin - 40 min
paraffin.



Until now we process these specimen with our standard (13 hours) protocol
and have no troubles with them. But I want to extend and improve fixation.



Thanks

Gudrun Lang

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