[Histonet] recommendation for skin and biopsy-processing

[Gudrun Lang]

Hi,

I want to implement a new processing protocol on the VIP 5 for biopsies and
skin. Please tell me your comments, if the protocol would be sufficient for
eg. 100 cassettes with biopsy-sponges and fifty cassettes with
skin-specimen. 

The program starts at 11 p.m., the specimen sit in NBF from afternoon till
the start.

30 min NBF - 30 min NBF - 30 min 70% - 30 min 80% - 30 min 96% - 40 min 96%
- 40 min 100% - 40 min 100% - 30 min shell sol (clearing medium) - 40 min
shell sol - 30 min Paraffin - 30 min paraffin - 40 min paraffin - 40 min
paraffin.

 

Until now we process these specimen with our standard (13 hours) protocol
and have no troubles with them. But I want to extend and improve fixation.

 

Thanks

Gudrun Lang