[Histonet] Bouin's fixative

[Geoff McAuliffe]

It is important to wash excess picrates out of the tissue after Bouin's 
fixation. Most people use several changes of 50% ethanol, one hour each. 
I suppose the "leaching buffer" is intended to do the same thing?

Geoff

Anthony Gatt wrote:
> Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative.  After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight.  What is the purpose of this buffer?
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************