[Histonet] Bouin's fixative

Anthony Gatt Anthony.Gatt <@t> jefferson.edu
Fri Sep 28 13:56:43 CDT 2007

Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative.  After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight.  What is the purpose of this buffer?

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