[Histonet] transfering tissue to another slide?

[John Kiernan]

The method on p. 69 of the AFIP manual is for covering some of the sections on a slide with an impervious material that prevents staining, so that you can apply two or more techniques to different sections on the same slide. It's not for removing and transferring sections.
 
Alkaline solutions are pretty good at removing sections from slides, and it's sometimes possible (with skill and luck) to remount them. Before trying, acidify and then rinse the section in water. Sections floating in an alkaline medium will not survive attempts to remove folds and creases.
 
John Kiernan
Anatomy, UWO
London, Canada
---
----- Original Message -----
From: Shawn Leslie <LeslieS <@t> vetmed.ufl.edu>
Date: Friday, September 28, 2007 11:05
Subject: Re: [Histonet] transfering tissue to another slide?
To: Thom Jensen <tissuearray <@t> hotmail.com>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

> We once used something called Diatex from Dow Corning.The 
> Address is on page 69 of the A.F.I.P. manual.I'm not sure if 
> they even sell anymore. That was a while ago....
> 
>  
> 
> Shawn Leslie
> Scientific Research Manager
> Anatomic Pathology
> University of Florida
> School of Veterinary Medicine
> 352-392-2235 ext 4555
> 
> 
> >>> Thom Jensen <tissuearray <@t> hotmail.com> 9/28/2007 10:08 AM >>>
> 
> Hey,
> Has anyone ever taken tissue off one slide and transfering it to 
> another?  I think I remember someone mentioning it in the past.
>  
> Anyone know anything?
>  
> Thanks,
> Thom
>  
>  
> for information on instructing TMA go to:  
> www.arrayworkshop.com 
>  
> New array instrument:  www.arraymold.com 
>  
>  
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