FW: [Histonet] transfering tissue to another slide?

[Ian Montgomery]

Terry,
	The tube of "stuff" was Durofix. Cast your mind back to when you
were a boy and built Airfix models, it's the same glue. Over the years I've
searched for Durofix but think it's no longer on the market. 
Technique.
1.) Remove the coverglass by soaking in warm xylene or equivalent.
2.) Soak in fresh clearing agent until you are sure the mounting media has
been removed. 
3.) Cover the section with a mixture of 6 parts butyl-acetate, 1 part
Durofix.
4.) Harden in a 37C oven for ~30 minutes.
5.) Carefully, with a sharp razor blade, cut the film from around the
section.
6.) Place the slide in cold water and leave until the film with the section
floats off.
7.) Mount on a clean slide.
8.) Drain excess water then place in a 37C oven until perfectly dry.
9.) Wash carefully with butyl-acetate.
10) Wash thoroughly in clearing agent and mount.

	When Durofix was available I used the technique on numerous
occasions and with a few tweeks it worked well. All you need do is find glue
that bonds plastics and you're away.
Ian. 


Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
G12 8QQ.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: 28 September 2007 15:30
To: Thom Jensen; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] transfering tissue to another slide?

Kemlo, 
We had a tube of "stuff" in Stoke which worked reasonably well. I have
not come across it since.
Remember the name?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thom
Jensen
Sent: 28 September 2007 15:09
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] transfering tissue to another slide?


Hey,
Has anyone ever taken tissue off one slide and transfering it to
another?  I think I remember someone mentioning it in the past.
 
Anyone know anything?
 
Thanks,
Thom
 
 
for information on instructing TMA go to:  www.arrayworkshop.com
 
New array instrument:  www.arraymold.com
 
 
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