[Histonet] mucin stain on matrigel-coated cultured cells

[Kim Merriam]

Hello,

Does anyone have a recommendation for a mucin stain for cultured cells on matrigel-coated slides?  An investigator at my site is interested in this.  I am worried that 1) the Schiff reagent will be too harsh for the cells and 2) that the matrigel itself might be PAS-positive.  I think IHC or IF might be the way to go, instead of a special stain.

Any thoughts?
Kim
 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA


       
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