[Histonet] Embedding polyurethane

[Phil McArdle]

Hello:

I'd have reservations about hexane - evil stuff. But as for IPA, 
assuming it's not India Pale Ale :-), that's a common shorthand for 
isopropyl alcohol (IUPAC 2-propanol), not to be confused with rubbing 
alcohol that's been watered down. I've had no experience with either 
hexane or IPA as an acetone replacement in this context, though.

Good luck!

Phil McArdle
-- 
Phil McArdle
Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel:  800.992.9037 x 341
Mobile: 860.597.6796
Fax: 860.653.0422

pmcardle <@t> ebsciences.com
www.ebsciences.com

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Boneslides <@t> aol.com wrote:
> Hello Histonetters!!
>  
> Does anyone know if hexane can be used in place of acetone?  I have a  series 
> of orthopedic specimens that contain a polyurethane implant that the  
> researcher wants embedded in Spurr's and then rough cut and ground.  I  used my usual 
> protocol, but the acetone caused the polyurethane to swell.   The company did 
> some research and was told that hexane could work and possibly  something 
> called IPA, as these are both considered non-solvents for  polyurethane.  I am 
> not familiar with either of these substances.  Any  one have any insight??
>  
> Any help would be much appreciated!
>  
> ~Diane
>  
>  
> Diane M.  Mahovlic, HT, MLT(ASCP)
> Orthopedic Pathology & Biomaterials  Laboratory
> Department of Anatomic Pathology
> The Cleveland Clinic  Foundation
> 
> 9500 Euclid Avenue- L30
> Cleveland, Ohio  44195
> 216-444-0166
> 
> 
> 
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-- 
Phil McArdle
Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel:  800.992.9037 x 341
Mobile: 860.597.6796
Fax: 860.653.0422

pmcardle <@t> ebsciences.com
www.ebsciences.com

I skate to where the puck is going to be, not to where it's been.
- Wayne Gretsky

You must be the change you want to see in the world.
- Mahatma Gandhi

NOTE: This message, together with any attachments, is intended only for 
the use of the individual or entity to which it is addressed and may 
contain information that is legally privileged, confidential and exempt 
from disclosure. If you are not the intended recipient, however, there's 
not a lot I can do about it, and it was probably my mistake anyway. So 
please do the right thing and make this e-mail go away. Thank you.