[Histonet] notch-1

[Sebree Linda A.]

Hi Valeria,

I have tried 2 of Santa Cruz's NOTCH-1 abs with no success on FFPE
sections (#s 6014 and 9170) but they don't claim to work for this
application or not with my system, i.e. raised in goat.  The one my
researcher DIDN'T buy is the 6014-R (raised in rabbit) that might have
actually worked with my system.  At my researcher's insistence, I did a
1:5 dilution, 10" HIER in a pressure cooker and amplification of the
primary ab.  All I got was nicely delineated epithelial cells against a
nasty brown background but no differentiation between normal and
malignant prostate cells, which was the focus of his research.

Good luck!.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Valeria
Berno
Sent: Tuesday, September 18, 2007 10:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] notch-1


Hello All,
I was wondering if there is anyone out there who has successfully done
Notch-1 staining on paraffin sections. If there is, would they be able
to 
could you tell me of the antibody, fixation/embedding protocol, and
subsequent 
treatments, if any. I have tried 3 Santa cruz antibodies unsuccessfully
and
we were wondering if it is something about the antibodies or the
protocol!!!
Thanks a lot
 
Valeria

Berno Valeria, PhD
 

EMBL Monterotondo Outstation

via Ramarini 32

00015 Monterotondo Scalo (RM)

Italy

Tel:  +39 06 90091 287

Fax: +39 06 90091 272

HYPERLINK "mailto:valeria.berno <@t> embl.it"valeria.berno <@t> embl.it

 

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