[Histonet] Golgi-Cox issue

[Liz Chlipala]

Lots of mounting media and the smallest coverslip possible, that way you =
have less chance for bubbles.  When we mounted whole rat corneas we =
placed the mounting media on top of the section and carefully placed the =
coverslip over the top of the specimen.  Then we used some nuts from the =
hardware store and placed them on the coverglass to flatten out the =
specimen and let dry overnight at room temp we found that heating the =
slides caused more air bubbles.

Liz=20

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
=20
Ship to Address:
=20
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of =
alonso.martinezcanabal <@t> utoronto.ca
Sent: Friday, September 14, 2007 11:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Golgi-Cox issue

   Hi,

         I am working with Golgi-Cox sections, at 120 microns. I =
everything is very good, except that I cannot mount the sections very =
well, apparently the tickness is causing troubles and the sections get =
dry-like some days after.

  Any sugiestions to mount with permount the tick sections?

   Thank Yoy very much



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